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Biochemical Genetics

, Volume 11, Issue 4, pp 301–308 | Cite as

An intergenic alcohol dehydrogenase isozyme in sunflowers

  • Andrew M. Torres
Article

Abstract

The isozymes of alcohol dehydrogenase (ADH; E.C. 1.1.1.1) in wild and cultivated sunflower (Helianthus annuus) seeds can be resolved electrophoretically into 12 bands. The slowest- and probably the fastest-migrating sets of three are allozymic products of two genes, Adh 1 and Adh 2 , each having two alleles, F (for fast) and S (for slow). Evidence from dissociation-recombination experiments utilizing bands excised from starch gels indicates that an intermediately-migrating isozyme is a dimeric intergenic product consisting of ADH-1F and ADH-2S subunits. The hybrid isozyme was unstable in vitro in that its monomers spontaneously dissociated and recombined to produce ADH-1FF and ADH-2SS isozymes. The molecular weights of the hybrid as well as the parental isozymes were estimated at approximately 98,000.

Key words

sunflowers alcohol dehydrogenase intergenic isozymes dissociation-recombination 

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Copyright information

© Plenum Publishing Corporation 1974

Authors and Affiliations

  • Andrew M. Torres
    • 1
  1. 1.Department of BotanyUniversity of KansasLawrence

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