Biochemical Genetics

, Volume 10, Issue 3, pp 297–307 | Cite as

Metabolic properties of erythrocytes of normal and genetically anemic mice

  • John J. Hutton
  • Seldon E. Bernstein


The murine hemolytic anemias microcytosis (gene symbol mk), normoblastosis (nb), spherocytosis (sph), and hemolytic (ha) are inherited as autosomal recessive diseases and resemble the human hereditary hemolytic anemias caused by defective enzyme activities in erythrocytes. The activities of 14 different enzymes of the glycolytic and pentose phosphate pathways were compared in erythrocytes from normal and anemic mice, but no quantitative differences suggesting enzyme deficiency were found. There were no major changes in reduced glutathione, NAD, NADP, or methemoglobin content. The rate of entry of glucose into the glycolytic and hexose monophosphate shunt pathways of intact erythrocytes was higher in mk/mk erythrocytes than predicted. Interpretation of studies of erythrocytes from anemic mice is generally complicated by the extremely high reticulocyte and nucleated cell counts in ha/ha, sph/sph, and nb/nb mice.


Glutathione Hexose Pentose Hemolytic Anemia Pentose Phosphate Pathway 
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Copyright information

© Plenum Publishing Corporation 1973

Authors and Affiliations

  • John J. Hutton
    • 1
  • Seldon E. Bernstein
    • 2
  1. 1.Department of MedicineUniversity of KentuckyLexington
  2. 2.The Jackson LaboratoryBar Harbor

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