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Biochemical Genetics

, Volume 5, Issue 6, pp 541–547 | Cite as

Micro-scale peptide mapping method for identification of variant proteins

  • Sumiko Watanabe
  • Akira Yoshida
Article

Abstract

A micro-scale (for protein of the order of 10−9 mole) peptide mapping method in thin-layer cellulose is reported. Peptide maps of tryptic digests from glucagon, from normal and variant β-chains of human hemoglobin, from human glucose 6-phosphate dehydrogenase, and from human phosphoglycerate kinase showed satisfactory resolution; thus this method can be used for characterizing the structure of variant enzymes and proteins of 10−9 molar quantity.

Keywords

Glucose Peptide Cellulose Glucagon Mapping Method 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Publishing Corporation 1971

Authors and Affiliations

  • Sumiko Watanabe
    • 1
  • Akira Yoshida
    • 1
  1. 1.Division of Medical Genetics, Department of Medicine, School of MedicineUniversity of WashingtonSeattle

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