Biochemical Genetics

, Volume 5, Issue 6, pp 541–547 | Cite as

Micro-scale peptide mapping method for identification of variant proteins

  • Sumiko Watanabe
  • Akira Yoshida


A micro-scale (for protein of the order of 10−9 mole) peptide mapping method in thin-layer cellulose is reported. Peptide maps of tryptic digests from glucagon, from normal and variant β-chains of human hemoglobin, from human glucose 6-phosphate dehydrogenase, and from human phosphoglycerate kinase showed satisfactory resolution; thus this method can be used for characterizing the structure of variant enzymes and proteins of 10−9 molar quantity.


Glucose Peptide Cellulose Glucagon Mapping Method 
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Copyright information

© Plenum Publishing Corporation 1971

Authors and Affiliations

  • Sumiko Watanabe
    • 1
  • Akira Yoshida
    • 1
  1. 1.Division of Medical Genetics, Department of Medicine, School of MedicineUniversity of WashingtonSeattle

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