Abstract
Actinomycin D at a concentration of 5 μg/ml of medium inhibited DNA-dependent RNA synthesis by 92% at 35 C, 42% at 30 C, and 28% at 25 C in Neurospora crassa. This concentration also inhibited the development of conidiophores and conidia at 35 C, but not at 30 or 25 C. Mycelia which were induced to synchronous development formed conidiophores in 2.5 hr and conidia in 4.5 hr at 35 C in the absence of drug additives. Addition of actinomycin D to synchronously developing mycelia at zero time and at 0.5-hr intervals thereafter at 35 C indicated that RNA synthesis required for conidiophores occurred before 0.5 hr and for conidia before 2 hr. Addition of cycloheximide at the same times to another synchronous mycelial series at 35 C indicated that protein synthesis required for conidiophores occurred before 2 hr and for conidia before 3.5–4 hr.
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References
Bollum, F. J. (1959). Thermal conversion of nonpriming deoxyribonucleic acid to primer. J. Biol. Chem. 234 2733.
Gornall, A. G., Bardawill, C. J., and David, M. M. (1949). Determination of serum proteins by means of the Biuret reaction. J. Biol. Chem. 177 751.
Gratzner, H., and Sheehan, D. N. (1969). Neurospora mutant exhibiting hyperproduction of amylase and invertase. J. Bacteriol. 97 544.
Hanks, D. L., and Sussman, A. S. (1969). Control of trehalase synthesis in Neurospora crassa. Am. J. Botany 56 1160.
Horowitz, N. H., Feldman, H. M., and Pall, P. L. (1970). Derepression of tyrosinase synthesis in Neurospora by cycloheximide, actinomycin D, and puromycin. J. Biol. Chem. 245 2784.
Kenney, F. T., Wicks, W. D., and Greenman, D. L. (1965). Hydrocortisone stimulation of RNA synthesis in induction of hepatic enzymes. J. Cellular Comp. Physiol. 66 125.
Kersten, W., Kersten, H., and Rauen, H. M. (1960). Action of nucleic acids on the inhibition of growth by actinomycin of Neurospora crassa. Nature 187 60.
Kirk, J. M. (1960). The mode of action of actinomycin D. Biochim. Biophys. Acta 42 167.
Loening, U. E. (1967). The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis. Biochem. J. 102 251.
McKeehan, W., and Hardesty, B. (1969). The mechanism of cycloheximide inhibition of protein synthesis in rabbit reticulocytes. Biochem. Biophys. Res. Commun. 36 625.
Melera, P. W., Chet, I., and Rusch, H. P. (1970). Electrophoretic characterization of ribosomal RNA from Physarum polycephalum. Biochim. Biophys. Acta 209 569.
Pall, M. L. (1966). The use of cycloheximide as an inhibitor of protein synthesis in Neurospora. Neurospora Newsletter 9 16.
Reich, E., Franklin, R. M., Shatkin, A. J., and Tatum, E. L. (1961). Effect of actinomycin D on cellular nucleic acid synthesis and virus production. Science 134 556.
Siegel, R. W., Matsuyama, S. S., and Urey, J. C. (1968). Induced macroconidia formation in Neurospora crassa. Experientia 24 1179.
Stine, G. J., and Clark, A. M. (1967). Synchronous production of conidiophores and conidia of Neurospora crassa. Can. J. Microbiol. 13 447.
Vogel, H. J. (1956). A convenient growth medium for Neurospora (medium N). Microbial Genet. Bull. 13 42.
Wells, R. D., and Larson, J. E. (1970). Studies on the binding of actinomycin D to DNA and DNA model polymers. J. Mol. Biol. 49 319.
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This work was supported in part by U.S. Public Health Service Training Grant 1 TO1 GM 01968 01.
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Totten, R.E., Howe, H.B. Temperature-dependent actinomycin D effect on RNA synthesis during synchronous development in Neurospora crassa . Biochem Genet 5, 521–532 (1971). https://doi.org/10.1007/BF00485670
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DOI: https://doi.org/10.1007/BF00485670