Abstract
A method has been developed for phenotyping group-specific component (Gc) by antibody-antigen crossed electrophoresis. The advantages of the method are that it increases the sensitivity of phenotyping, especially when Gc is partly degraded, and that it may permit quantitative genetic studies to be carried out. Concentrated amniotic fluid was found to be an ideal antigen for obtaining a multivalent rabbit antiserum with high titres of anti-Gc antibodies.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bearn, A. G., Kitchin, F. D., and Bowman, B. H. (1964). Heterogeneity of the inherited group-specific component of human serum. J. Exptl. Med. 12083.
Derrington, M. M., and Soothill, J. F. (1961). An immunochemical study of the proteins of amniotic fluid and of maternal and foetal serum. J. Obstet. Gynaecol. Brit. Cmwlth. 68755.
Freeman, T., and Smith, J. (1970). Human serum protein fractionation by gel filtration. Biochem. J. 118869.
Hirschfeld, J. (1959). Immune-electrophoretic demonstration of qualitative differences in human sera and their relation to the haptoglobins. Acta Pathol. Microbiol. Scand. 47160.
Hirschfeld, J. (1960). Immunoelectrophoresis—Procedure and application to the study of group-specific variations in sera. Sci. Tools 718.
Kitchin, F. D. (1965). Demonstration of the inherited serum group specific protein by acrylamide electrophoresis. Proc. Soc. Exptl. Biol. Med. 1191153.
Kitchin, F. D., and Bearn, A. G. (1966). The electrophoretic patterns of normal and variant phenotypes of the group-specific (Gc) components in human serum. Am. J. Hum. Genet. 18201.
Laurell, C. B. (1965). Antigen-antibody crossed electrophoresis. Ann. Biochem. 10358.
Nerstrøm, B. (1963). Experimental transformation of the group-specific components (Gc) of serum into a single alpha-1 globulin immunologically identical with the Gc. Acta Pathol. Microbiol. Scand. 57495.
Nerstrøm, B., Mansa, B., and Frederiksen, W. (1964). Alteration of the Gc patterns in human sera incubated with bacteria. Acta Pathol. Microbiol. Scand. 61474.
Reinskou, N. (1963). A heterogeneity of the fast moving component of the Gc system. Acta Pathol. Microbiol. Scand. 59526.
Schultz, H. E., and Heremens, J. F. (1966). Molecular Biology of Human Proteins, Vol. I, Elsevier, Amsterdam.
Sutcliffe, R. G., and Brock, D. J. H. (1973). J. Obstet. Gynaecol. Brit. Cmwlth., in press.
Sutcliffe, R. G., Brock, D. J. H., and Scrimgeour, J. B. (1972). The origin of amniotic fluid group-specific component. Nature 238400.
Usategui-Gomez, M., Morgan, D. F., and Toolan, H. W. (1966). A comparative study of amniotic fluid, maternal sera and cord sera by disc electrophoresis. Proc. Soc. Exptl. Biol. Med. 123547.
von Kleist, S., Buffe, D., and Burtin, P. (1968). Relation entre les antigènes du liquide aniotique et du sérum foetal. Clin. Chim. Acta 2089.
Weeke, B. (1970). The serum proteins identified by means of the Laurell crossed electrophoresis. Scand. J. Clin. Lab. Invest. 25269.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sutcliffe, R.G., Brock, D.J.H. Group-specific component phenotyping by antibody-antigen crossed electrophoresis. Biochem Genet 9, 63–68 (1973). https://doi.org/10.1007/BF00485591
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00485591