Genetic variation in the carbonic anhydrase isozymes of macaque monkeys. I. The radioimmunosorbent assay
- 13 Downloads
A radioimmunosorbent technique is described which is capable of independently detecting both isozymes of carbonic anhydrase, CA I and CA II, in concentrations as low as 1 ng/ml. The technique is used to quantitate the different electrophoretic variants of red cell CA I as well as levels of CA II in the pig-tailed macaque, Macaca nemestrina.
KeywordsGenetic Variation Carbonic Anhydrase Macaque Monkey Electrophoretic Variant Carbonic Anhydrase Isozyme
Unable to display preview. Download preview PDF.
- DeSimone, J., Magid, E., and Tashian, R. E. (1973). Genetic variation in the carbonic anhydrase isozymes of macaque monkeys. II. Inheritance of red cell carbonic anhydrase levels in different carbonic anhydrase I genotypes of the pig-tailed macaque, Macaca nemestrina. Biochem. Genet. 8165.Google Scholar
- Headings, V. E., and Tashian, R. E. (1970). A radioimmunoassay for quantifying carbonic anhydrase isozymes in crude lysates. Biochem. Genet. 4285.Google Scholar
- Headings, V. E., and Tashian, R. E. (1971). Quantitative genetic variation in carbonic anhydrase isozymes from tissues of the pig-tailed macaque, Macaca nemestrina. Biochem. Genet. 5333.Google Scholar
- Kampen, E. J., and Zijlstra, W. G. (1965). Determination of hemoglobin and its derivatives. Advan. Clin. Chem. 8141.Google Scholar
- Magid, E. (1967). The activity of carbonic anhydrases B and C from human erythrocytes and the inhibition of the enzymes by copper. Scand. J. Haematol. 4257.Google Scholar
- Tanis, R. J., Tashian, R. E., and Yu, Y.-S. L. (1970). Properties of carbonic anhydrase isozymes isolated from porcine erythrocytes. J. Biol. Chem. 2456003.Google Scholar
- Tashian, R. E. (1969). The esterases and the carbonic anhydrases of human erythrocytes. In Yunis, J. J. (ed.), Biochemical Methods in Red Cell Genetics, Academic Press, New York, p. 307.Google Scholar
- Tashian, R. E., Goodman, M., Headings, V. E., DeSimone, J., and Ward, R. H. (1971). Genetic variation and evolution in the red cell carbonic anhydrase isozymes of macaque monkeys. Biochem. Genet. 5183.Google Scholar
- Wide, L. (1969). Radioimmunoassays employing immunosorbents. Acta Endocrinol. 63207 (suppl. 142).Google Scholar