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Identification and genetic control of starch-degrading enzymes in maize endosperm

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Abstract

Three starch-degrading enzymes from liquid endosperm of maize have been separated by means of horizontal acrylamide gel electrophoresis. The three enzymes are tentatively identified as α-amylase (zone 1), β-amylase (zone 2), and α-glucan phosphorylase (zone 3). Electrophoretic variants of these enzymes were found among ten inbred strains examined. Results of genetic crosses with respect to zone 2 amylase show that it is controlled by a pair of alleles (Amy-2 A and Amy-2 B) acting without dominance. It further appears that Amy-2 and Ct (catalase) are linked with 5% recombination frequency.

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Authors and Affiliations

  1. MSU/AEC Plant Research Laboratory, Michigan State University, East Lansing, Michigan

    Su En Chao & John G. Scandalios

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  1. Su En Chao
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  2. John G. Scandalios
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Additional information

This work was supported by the U.S. Atomic Energy Commission, under contract No. AT(11-1)-1338.

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Chao, S.E., Scandalios, J.G. Identification and genetic control of starch-degrading enzymes in maize endosperm. Biochem Genet 3, 537–547 (1969). https://doi.org/10.1007/BF00485475

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  • DOI: https://doi.org/10.1007/BF00485475

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