Biochemical Genetics

, Volume 13, Issue 9–10, pp 585–602 | Cite as

Rapidly labeled proteins on the salivary gland chromosomes of Drosophila melanogaster

  • Herschel K. Mitchell
  • Loveriza S. Lipps
Article

Abstract

Experiments on short-term and pulse-chase labeling of chromosome proteins of the salivary glands of Drosophila melanogaster show unique patterns of label in the vicinity of chromosome puffs. A high turnover rate is indicated for these nonhistone proteins, which appear to form a fibrous sheath around the chromosomes. Acrylamide gel analyses of the chromosomal proteins that are quickly labeled, comparing compositions at different stages of development with compositions after heat shock, show that all are different and dependent on which chromosomal puffs are active and producing messenger RNA. The necessity for a continuous and rapid interchange of protein between the nucleus and cytoplasm is indicated, and it appears that regulation of gene activity must be related to this dynamic state of protein exchange. From the technical standpoint, it has been found that scanning electron microscopy (SEM) is especially useful for observing silver grains on opaque autoradiographs. It appears also that SEM will prove useful in a variety of studies of chromosome structure.

Key words

salivary glands nuclei nonhistone proteins puffs chromosomal proteins 

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References

  1. Ashburner, M. (1967). Patterns of puffing activity in the salivary gland chromosome of Drosophila. I. Autosomal puffing patterns in a laboratory stock of Drosophila melanogaster. Chromosoma 21398.Google Scholar
  2. Ashburner, M. (1970). Patterns of puffing activity in the salivary gland chromosome of Drosophila. II. Responses to environmental treatments. Chromosoma 31356.Google Scholar
  3. Berendes, H. D. (1968). Factors involved in the expression of gene activity in polytene chromosomes. Chromosoma 24418.Google Scholar
  4. Cave, D. M. (1968). Chromosome replication and synthesis of non-histone proteins in giant polytene chromosomes. Chromosoma 25392.Google Scholar
  5. Clever, U. (1964). Actinomycin and puromycin: Effects on sequential gene activation by ecdysone. Science 146794.Google Scholar
  6. Clever, U. (1967). In Goldstein, L. (ed.), The Control of Nuclear Activity, Prentice-Hall, Englewood Cliffs, N.J., 24:161.Google Scholar
  7. Dice, F. J., and Schimke, R. T. (1973). Turnover of chomosomal proteins from rat liver. Arch. Biochem. Biophys. 15897.Google Scholar
  8. Gallwitz, D., and Mueller, G. C. (1969). Histone synthesis in vitro on hela cell microsomes. The nature of the coupling to deoxyribonucleic acid synthesis. J. Biol. Chem. 2445947.Google Scholar
  9. Hodges, G. M., Carbonell, A. W., Muir, M. D., and Grant, P. R. (1974). Uses and limitations of scanning electron microscope autoradiography. Scanning electron microscopy. Part I. In Johari O., and Corvin, I. (eds.), Proceedings of the Seventh Annual Scanning Electron Microscope Symposium, IIT Research Institute, Chicago, Illinois, pp. 159–164.Google Scholar
  10. Kay, D., ed. (1961). Techniques for Electron Microscopy, Blackwell Scientific Publications, Oxford, pp. 122–131.Google Scholar
  11. Kay, D., ed. (1965). Techniques for Electron Microscopy, Blackwell Scientific Publications, Oxford, pp. 529–533.Google Scholar
  12. Mitchell, H. K., Weber-Tracy, U., and Schaar, G. (1971). Aspects of cuticle formation in Drosophila melanogaster. J. Exp. Zool. 176429.Google Scholar
  13. Oliver, D. R., and Chalkley, R. (1972). An electrophoretic analysis of Drosophila histones Exp. Cell Res. 73303.Google Scholar
  14. Pelling, C. (1964). Ribonukleinsaure-synthese der riesenchromosomen. Autoradiographische untersuchungen an chironomus tentans. Chromosoma 1571.Google Scholar
  15. Pettit, B. J., and Rasch, R. W. (1966). Tritiated-histidine incorporation in the Drosophila salivary chromosomes. J. Cell Physiol. 68325.Google Scholar
  16. Rudkin, G. T. (1973). In Balls, M., and Billett, F. S. (eds.), The Cell Cycle in Development and Differentiation, Syndics of the University Press, London.Google Scholar
  17. Scheid, W., Traut, H., and Pfautsch, M. (1972). Visualization by scanning electron microscope of giant Chomosomes of Drosophila melanogaster. Drosophila Inform. Serv. 4839.Google Scholar
  18. Tissieres, A., Mitchell, H. K., and Tracy, U. (1974) Protein synthesis in salivary glands of Drosophila melanogaster: Relation to chromosome puffs. J. Mol. Biol. 84389.Google Scholar

Copyright information

© Plenum Publishing Corporation 1975

Authors and Affiliations

  • Herschel K. Mitchell
    • 1
  • Loveriza S. Lipps
    • 1
  1. 1.Division of BiologyCalifornia Institute of TechnologyPasadena

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