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Glucose 6-phosphate dehydrogenase in rainbow trout

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Abstract

Electrophoretic analysis of glucose 6-phosphate dehydrogenase from liver and blood of rainbow trout revealed a complex series of bands, which could differ between fish. The partial interconvertible nature of these bands was demonstrated with enzyme that had been incompletely inactivated at pH 8.4. In a single population of 40 fish, a homozygote and a heterozygote for an electrophoretic variant allele were found. We suggest that G6PD in rainbow trout liver and blood is determined by two alleles at a single locus, with posttranslational modification responsible for the complex electrophoretic patterns seen. The basis for this variation appears to be NADH binding to the protein molecule. Another variant and other properties of the enzyme are described.

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Authors and Affiliations

  1. Departments of Medicine and Genetics, University of Washington, Seattle, Washington

    Stephen D. Cederbaum & Akira Yoshida

  2. Department of Biochemical Genetics, City of Hope National Medical Center, Duarte, California

    Akira Yoshida

Authors
  1. Stephen D. Cederbaum
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  2. Akira Yoshida
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Additional information

Supported in part by the State of California Department of Mental Hygiene and by USPHS Grants GM-15253, HD-04612, HD-00315, HD-05615, and HL-15125. One of us (S.D.C.) was a Special Postdoctoral Fellow of NIAMD (No. 1F03AM-40, 329-01) during part of this work.

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Cederbaum, S.D., Yoshida, A. Glucose 6-phosphate dehydrogenase in rainbow trout. Biochem Genet 14, 245–258 (1976). https://doi.org/10.1007/BF00484764

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  • DOI: https://doi.org/10.1007/BF00484764

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