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Biochemical Genetics

, Volume 15, Issue 1–2, pp 195–210 | Cite as

Biochemical genetics of Chinese hamster cell mutants with deviant purine metabolism. VI. Enzymatic studies of two mutants unable to convert inosinic acid to adenylic acid

  • Alice S. Tu
  • David Patterson
Article

Abstract

AdeH and adeI are two auxotrophic mutants of Chinese hamster ovary (CHO-K1) cells which specifically require adenine as the purine source to grow. The enzymatic defects of these mutants were examined in cell-free extracts. It was found that adeH did not have any detectable adenylosuccinate synthetase activity and adeI was defective in the adenylosuccinate lyase enzyme. The relevance of adenine-requiring mutants to the study of the regulation of purine metabolism in mammalian cells is discussed.

Key words

CHO-K1 cells adenine-requiring mutants adenylosuccinate synthetase adenylosuccinate lyase 

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Copyright information

© Plenum Publishing Corporation 1977

Authors and Affiliations

  • Alice S. Tu
    • 1
  • David Patterson
    • 1
  1. 1.Eleanor Roosevelt Institute for Cancer Research, and the Department of Biophysics and GeneticsUniversity of Colorado Medical CenterDenver

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