Abstract
Three alleles at the Gpt-1 (glutamic-pyruvic transaminase-1) locus in the mouse, as identified by electrophoresis on cellulose acetate, and their distribution among inbred mouse strains and wild stocks are described. The Gpt-1 locus was shown to control the soluble form of the enzyme. Three-point linkage analysis established the location of Gpt-1 on chromosome 15 between uw and bt. In addition, a new staining procedure is described that allows the visualization of GPT activity on gels by the deposition of formazan. This is an improvement over previous methods that produced bands of nonfluorescence against a fluorescent background.
Similar content being viewed by others
References
Chen, S.-H., and Giblett, E. R. (1971). Polymorphism of soluble glutamic-pyruvic transaminase: A new genetic marker in man. Science 173148.
Chen, S.-H., Giblett, E. R., Anderson, J. E., and Fossum, B. L. G. (1972). Genetics of glutamic-pyruvic transaminase: Its inheritance, common and rare variants, population distribution, and differences in catalytic activity. Ann. Hum. Genet. 35401.
Chen, S.-H., Donahue, R. P., and Scott, C. R. (1973). The genetics of glutamic-pyruvic transaminase in mice: Inheritance, electrophoretic phenotypes, and postnatal changes. Biochem. Genet. 1023.
Mouse News Letter (1975). Listing of mouse genes. 527.
Ragab, H., Beck, C., Dillard, C., and Tappel, A. L. (1967). Preparation of rat liver lysosomes. Biochim. Biophys. Acta 148501.
Swick, R. W., Barnstein, P. L., and Stange, J. L. (1965). The metabolism of mitochondrial proteins. I. Distribution and characterization of the isozymes of alanine aminotransferase in rat liver. J. Biol. Chem. 2403334.
Author information
Authors and Affiliations
Additional information
This investigation was supported in part by Research Grant GM 20919 from the National Institute of General Medical Sciences, and by contract NO1-ES-4-2159 with the National Institute of Environmental Health Sciences. The Jackson Laboratory is fully accredited by the American Association for Accreditation of Laboratory Animal Care.
Rights and permissions
About this article
Cite this article
Eicher, E.M., Womack, J.E. Chromosomal location of soluble glutamic-pyruvic transaminase-1 (Gpt-1) in the mouse. Biochem Genet 15, 1–8 (1977). https://doi.org/10.1007/BF00484544
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00484544