Abstract
UMPK 3 is a rare variant of the polymorphic enzyme of human red cells, uridine monophosphate kinase. This homozygote phenotype was detected among the Warao Indians of Venezuela. The UMPK 1 and UMPK 3 enzymes were partially purified following the method described by Tend et al. (1976). The biochemical and kinetic parameters of both variants were studied in crude hemolysates and in partially purified enzymes. A comparison was made with the results previously reported by Teng for UMPK 1 and UMPK 2, and it was concluded that UMPK 3 seems to resemble the other two allelic gene products in Km values for UMP, CMP, and ATP but differs from them in electrophoretic mobility, pH optimum, and thermal stability.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Beutler, E. (1971). Red Cell Metabolism: A Manual of Biochemical Methods Grune & Stratton, New York.
Fildes, R. A., and Harris, H. (1966). Genetically determined variation of adenylate kinase in man. Nature 209261.
Gallango, M. L., and Suinaga, R. (1978). Uridine monophosphate kinase polymorphism in two Venezuelan populations. Am. J. Hum. Genet. 30215.
Giblett, E. R., Anderson, J. E., Chen, S. H., Teng, Y. S., and Cohen, F. (1974). Uridine monophosphate kinase: A new genetic polymorphism with possible clinical implications. Am. J. Hum. Genet. 26627.
Lineweaver, H., and Burk, D. (1934). The determination of enzyme dissociation constants. J. Am. Chem. Soc. 55658.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. (1951). Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193265.
Teng, Y. S., Chen, S. H., and Scott, R. (1976). Human erythrocyte pyrimidine nucleoside monophosphate kinase: Partial purification and properties of two allelic gene products. J. Biol. Chem. 2514179.
Author information
Authors and Affiliations
Additional information
Unidad de Hematología y Oncología, M.S.A.S.
Rights and permissions
About this article
Cite this article
Gallango, M.L., Müller, A. & Suinaga, R. Biochemical characterization of a red cell UMP kinase variant found in the Warao Indians of Venezuela. Biochem Genet 16, 1085–1092 (1978). https://doi.org/10.1007/BF00484529
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00484529