Abstract
The proteins secreted by the rat seminal vesicle can be separated into five major fractions (namely, RSV-I through V) by gel electrophoresis in denaturing conditions. Two polymorphic proteins, svp-1 and svp-2, also present in the mouse, are produced by the seminal vesicle as well, but the procedure used for their identification makes it impossible to ascertain whether they correspond to any of the major fractions mentioned above. We show here that, on the basis of molecular weight measurements and of amino acid composition determinations, svp-1 and RSV-V are indeed the same protein. We also show that svp-2 is strictly related to another major secretory protein, RSV-IV, whose amino acid composition is almost identical, but for a few amino acid residues, to that of svp-2. We thus conclude that the latter protein is a variant of RSV-IV that can be expressed only in rats homozygous for a given allele at the svp-2 locus. This paper thus brings together published information on the genetics of the loci coding for svp-1 and for svp-2 and on the molecular biology of RSV-IV and RSV-V and of their corresponding gene.
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This work was supported by a CNR grant from Progetto Finalizzato Ingegneria Genetica e Basi Molecolari delle Malattie genetiche.
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Maffei, A., Paonessa, G., Abrescia, P. et al. Polymorphism of rat seminal vesicle secretory proteins: Characterization of svp-1 and svp-2 and their identification with the major secretory proteins IV and V. Biochem Genet 22, 567–576 (1984). https://doi.org/10.1007/BF00484523
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DOI: https://doi.org/10.1007/BF00484523