Biochemical Genetics

, Volume 15, Issue 11–12, pp 1133–1142 | Cite as

Nonidentical subunits of human erythrocyte phosphofructokinase

  • Jasminder Kaur
  • Robert B. Layzer
Article

Abstract

Human erythrocyte and muscle phosphofructokinase (PFK) were purified completely by improved procedures. SDS-acrylamide gel electrophoresis in a discontinuous buffer system revealed two subunits (R and M) of erythrocyte PFK, the slower one (M) corresponding to the single subunit of muscle PFK. The staining intensity ratio R:M of the two bands of erythrocyte PFK was 2:1 or less. This suggests that native erythrocyte PFK contains multiple isoenzymes with different proportions of R and M, some being lost during purification. Nevertheless, isoelectric focusing showed single peaks of erythrocyte PFK (pI 5.0) and muscle PFK (pI 6.6), perhaps because of aggregation of erythrocyte PFK isoenzymes. Erythrocyte PFK from a patient with muscle PFK deficiency had a pI of 4.6 and could not be precipitated by antiserum against muscle PFK, findings compatible with the putative structure R4.

Key words

phosphofructokinase inborn error of metabolism isoenzymes enzyme subunits 

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Copyright information

© Plenum Publishing Corporation 1977

Authors and Affiliations

  • Jasminder Kaur
    • 1
  • Robert B. Layzer
    • 1
  1. 1.Department of NeurologyUniversity of California, School of MedicineSan Francisco

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