Biochemical Genetics

, Volume 15, Issue 3–4, pp 273–286 | Cite as

Nuclear and cytoplasmic genome relationships in the genus Avena: Analysis by isoelectric focusing of ribulose biphosphate carboxylase subunits

  • M. W. Steer
  • D. Kernoghan
Article

Abstract

Comparisons of the isoelectric points of small and large subunits of ribulose biphosphate carboxylase extracted from a number of diploid, tetraploid, and hexaploid Avena species have been used to obtain information on the nuclear and cytoplasmic genome relationships within the genus. All species tested had small subunits with similar isoelectric points, so their analysis provided no information of taxonomic value. Three types of large subunits could be distinguished by this method, and the distribution of each among the available species provides strong evidence against the involvement of a C genome diploid (such as A. ventricosa) as the maternal parent in the formation of either tetraploid or hexaploid species. One type of large subunit was confined to the perennial tetraploid, A. macrostachya, and its position in the genus and possible origin are discussed. The value of this approach in studying genome relationships within the genus Avena and related genera is assessed.

Key words

Avena cytoplasmic genome isoelectric focusing nuclear genome ribulose biphosphate carboxylase 

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References

  1. Baker, T. S., Eisenberg, D., Eiserling, F. A., and Weissmann, L. (1975). The structure of form 1 crystals of d-ribulose-1,5-diphosphate carboxylase. J. Mol. Biol. 91391.Google Scholar
  2. Baum, B. R. (1968). Delimitation of the genus Avena (Gramineae). Can. J. Bot. 46121.Google Scholar
  3. Baum, B. R. (1974). Classification of the oat species (Avena, Poaceae) using various taximetric methods and an information-theoretic model. Can. J. Bot. 522241.Google Scholar
  4. Baum, B.R., Rajhathy, T., and Sampson, D. R. (1973). An important new diploid Avena species discovered on the Canary Islands. Can. J. Bot. 51759.Google Scholar
  5. Chen, K., Gray, J. C., and Wildman, S. G. (1975). Fraction 1 protein and the origin of polyploid wheats. Science 1901304.Google Scholar
  6. Chen, K., Kung, S. D., Gray, J. C., and Wildman, S. G. (1976). Subunit polypeptide composition of fraction 1 protein from various plant species. Plant Sci. Lett. 7429.Google Scholar
  7. Cobb, A. H., and Wellburn, A. R. (1976). Polypeptide binding to plastid envelopes during chloroplast development. Planta 129127.Google Scholar
  8. Craig, I. L., Murray, B. E., and Rajhathy, T. (1974). Avena canariensis: Morphological and electrophoretic polymorphism and relationship to the A. magna-A. murphyi complex and A. sterilis. Can. J. Genet. Cytol. 16677.Google Scholar
  9. Gray, J. C., and Kekwick, R. G. O. (1974). An immunological investigation of the structure and function of ribulose 1,5-biphosphate carboxylase. Eur. J. Biochem. 44481.Google Scholar
  10. Gray, J. C., and Wildman, S. G. (1976). A specific immunoabsorbant for the isolation of fraction 1 protein Plant Sci. Lett. 691.Google Scholar
  11. Gray, J. C., Kung, S. D., Wildman, S. G., and Sheen, S. J. (1974). Origin of Nicotiana tabacum L. detected by polypeptide composition of fraction 1 protein. Nature 252226.Google Scholar
  12. Gunning, B. E. S. (1965). The fine structure of chloroplast stroma following aldehydeosmium tetroxide fixation J. Cell. Biol. 2479.Google Scholar
  13. Gunning, B. E. S., Steer, M. W., and Cochrane, M. P. (1968). Occurrence, molecular structure, and induced formation of the “stromacentre” in plastids. J. Cell Sci. 3445.Google Scholar
  14. Holden, J. H. W. (1966). Species relationships in the Avenae. Chromosoma 2075.Google Scholar
  15. Kawashima, N., and Wildman, S. G. (1972). Studies in fraction 1 protein. IV. Mode of inheritance of primary structure in relation to whether chloroplast or nuclear DNA contains the code for a chloroplast protein. Biochim. Biophys. Acta 26242.Google Scholar
  16. Kung, S. D. (1976). Tobacco fraction 1 protein: A unique genetic marker. Science 191429.Google Scholar
  17. Kung, S. D., Sakano, K., and Wildman, S. G. (1974). Multiple peptide composition of the large and small subunits of Nicotiana tabacum fraction 1 protein ascertained by fingerprinting and electrofocusing. Biochim. Biophys. Acta 365138.Google Scholar
  18. Kung, S. D., Gray, J. C., Wildman, S. G., and Carlson, P. S. (1975). Polypeptide composition of fraction 1 protein from parasexual hybrid plants in the genus Nicotiana. Science 187353.Google Scholar
  19. Ladizinsky, G. (1969). New evidence on the origin of the hexaploid oats. Evolution 23676.Google Scholar
  20. Ladizinsky, G. (1971a). Avena murphyi: A new tetraploid species of oat from southern Spain. Israel J. Bot. 2024.Google Scholar
  21. Ladizinsky, G. (1971b). Chromosome relationships between tetrapolid (2n=28) Avena murphyi and some diploid, tetraploid and hexaploid species of oats. Can. J. Genet. Cytol. 13203.Google Scholar
  22. Ladizinsky, G. (1974). Cytogenetic relationships between the diploid oat A. prostrata and the tetraploids A. barbata, A. magna and A. murphyi. Can. J. Genet. Cytol. 16105.Google Scholar
  23. Ladizinsky, G., and Zohary, D. (1967). Avena ventricosa: Possible diploid contributor to hexaploid oats. Science 1551553.Google Scholar
  24. Ladizinsky, G., and Zohary, D. (1971). Notes on species delimination, species relationships and polyploidy in Avena L. Euphytica 20380.Google Scholar
  25. Rajhathy, T. (1966). Evidence and an hypothesis for the origin of the C genome of hexaploid Avena. Can. J. Genet. Cytol. 8774.Google Scholar
  26. Rajhathy, T., and Morrison, J. W. (1959). Chromosome morphology in the genus Avena. Can. J. Bot. 37331.Google Scholar
  27. Rajhathy, T., and Sadasivaiah, R. S. (1969). The cytogenetic status of Avena magna. Can. J. Genet. Cytol. 1177.Google Scholar
  28. Rutner, A. C., and Lane, D. L. (1967). Nonidentical subunits of ribulose diphosphate carboxylase. Biochem. Biophys. Res. Commun. 28531.Google Scholar
  29. Sadasivaiah, R. S., and Rajhathy, T. (1968). Genome relationships in tetraploid Avena. Can. J. Genet. Cytol. 10655.Google Scholar
  30. Steer, M. W. (1975). Evolution in the genus Avena: Inheritance of different forms of ribulose diphosphate carboxylase. Can. J. Genet. Cytol. 17337.Google Scholar
  31. Steer, M. W., and Thomas, H. (1976). Evolution of Avena sativa: Origin of the cytoplasmic genome. Can. J. Genet. Cytol. 18769.Google Scholar
  32. Steer, M. W., Gunning, B. E. S., Graham, T. A., and Carr, D. J. (1968). Isolation, properties, and structure of fraction 1 protein from Avena sativa L. Planta 79254.Google Scholar
  33. Steer, M. W., Holden, J. H. W., and Gunning, B. E. S. (1970). Avena chloroplasts: Species relationships and the occurrence of stromacentres. Can. J. Genet. Cytol. 1221.Google Scholar
  34. Swami, U. B. S., and Thomas, H. (1966). Studies on the relationships of Avena sativa (6 ×) and Avena hirtula (2 ×). Can. J. Genet. Cytol. 8 50.Google Scholar
  35. Thomas, H. (1968). The addition of single chromosomes of Avena hirtula to the cultivated hexaploid oat A. sativa. Can. J. Genet. Cytol. 10551.Google Scholar
  36. Thomas, H. (1970). Chromosome relationships between the cultivated oat Avena sativa (6 ×) and A. ventricosa (2 ×). Can. J. Genet. Cytol. 1236.Google Scholar
  37. Thomas, H., and Leggett, J. M. (1974). Chromosome relationships between Avena sativa and the two diploid species A. canariensis and A. prostrata. Can. J. Genet. Cytol. 16889.Google Scholar
  38. Thomas, H., and Rajhathy, T. (1967). Chromosome relationships between Avena sativa (6×) and Avena pilosa (2×). Can. J. Genet. Cytol. 9154.Google Scholar
  39. Weber, K., and Osborne, M. (1969). The reliability of molecular weight determinations by dodecyl sulphate-polyacrylamide gel electrophoresis. J. Biol. Chem. 2444406.Google Scholar

Copyright information

© Plenum Publishing Corporation 1977

Authors and Affiliations

  • M. W. Steer
    • 1
  • D. Kernoghan
    • 1
  1. 1.Department of BotanyThe Queen's University of BelfastBelfastNorthern Ireland

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