Abstract
The esterases of the cotton boll weevil were separated by polyacrylamide gel electrophoresis into four major regions. These were named Est I–IV in order of migration from anode to origin. Polymorphism was observed in all regions. The Est II region was shown to consist of no more than two bands (fast and slow). The inheritance of the fast and slow bands of Est II was demonstrated to be controlled by codominant autosomal alleles. Analysis of the gene frequency of the Est II region showed that one field population was consistent with the Hardy-Weinberg law (P=0.995), while a second field population was not at equilibrium (P<0.001).
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Bull, D. L., and Lindquist, D. A. (1968). Cholinesterase in boll weevils, Anthonomus grandis Boheman. I. Distribution and some properties of the crude enzyme. Comp. Biochem. Physiol. 25639.
E. C. Apparatus Corporation (1966). Buffer and Enzyme Incubation Recipes, Technical Bulletin 135.
Gast, R. T. (1966). Oviposition and fecundity of boll weevils in mass-rearing cultures. J. Econ. Entomol. 59173.
Peacock, A. C., Bunting, S. L., and Queen, K. G. (1965). Serum protein electrophoresis in acrylamide gel: Patterns from normal human subjects. Science 1471451.
Peck, C. T., and Biggers, C. J. (1975). Electrophoretic analysis of plasma proteins of Mississippi Peromyscus. J. Hered. 66327.
Pollard, G. M., Biggers, C. J., and Harvey, M. J. (1973). Electrophoretic differentiation of the parotid venoms of Bufo americanus americanus and Bufo woodhousei foweleri. Herpetologica 29251.
Shaw, C. R. (1965). Electrophoresis variation in enzymes. Science 149936.
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This work was supported in part by a Faculty Research Grant from Memphis State University.
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Biggers, C.J., Bancroft, H.R. Esterases of laboratory-reared and field-collected cotton boll weevils, Anthonomus grandis Boh.: Polymorphism of adult esterases and formal genetics of esterase II. Biochem Genet 15, 227–233 (1977). https://doi.org/10.1007/BF00484455
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DOI: https://doi.org/10.1007/BF00484455