Abstract
Electrophoretic profiles of acid-extractable proteins from Holtzman rat liver chromatin display four minor and five major histone bands through certain stages of postweaned development but are qualitatively different from the chromatin protein profiles previously reported during postweaned development for the Fisher 344 rat strain and the F344 × Holtzman heterotic progeny [Tallman, G., et al. (1979). Biochem. Genet. 17:185]. The protein profiles from the heterotic progeny do not reflect and are not combinations of the profiles from the parental strains. Levels of in vitro transcription with Escherichia coli RNA polymerase of total chromatin and of acid-extracted chromatin from Holtzman rat liver tissue fluctuate in an age-specific manner during postweaned development and are higher than previously published levels determined for the Fisher and F344 × H strains during the same developmental period [Tallman, G., et al. (1978). J. Hered. 69:282]. The degrees of stimulation in the transcription assays resulting from the acid treatment vary with the age of the animal but are similar for the maternal Holtzman and hybrid strains. These studies suggest that regulation of heterotic growth may involve dominant, or maternal genetic influences.
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Amero, S. (1979). Dissertation, West Virginia University, Morgantown.
Bonner, J., Chalkley, R., Dahmus, M., Fambrough, D., Fujimura, F., Huang, R., Huberman, J., Hensen, R., Marushige, K., Ohlenbusch, H., Olivera, B., and Widholm, J. (1968). Isolation and characterization of chromosomal nucleoproteins. In Grossman, L., and Moldave, K. (eds.), Methods in Enzymology XIIB: Nucleic Acids Academic Press, New York, pp. 3–41.
Burton, K. (1968). Determination of DNA concentrations with diphenylamine. In Grossman, L., and Moldave, K. (eds.), Methods in Enzymology XIIB: Nucleic Acids Academic Press, New York, pp. 163–166.
Chong, M. T., Garrard, W. T., and Bonner, J. (1974). Purification and properties of a neutral protease from rat liver chromatin. Biochemistry 135128.
Davie, J. R., and Candido, E. P. M. (1978). Acetylated histone H4 is preferentially associated with template-active chromatin. Proc. Natl. Acad. Sci. USA 753574.
Epperly, M. (1980). Dissertation, West Virginia University, Morgantown.
Garrard, W. T., and Bonner, J. (1974). Changes in chromatin proteins during liver regeneration. J. Biol. Chem. 2495570.
Kota, D. (1977). Dissertation, West Virginia University, Morgantown.
Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 277680.
Lowry, O. H., Rosebrough, J. J., Farr, A. L., and Randall, R. J. (1951). Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193365.
Manwell, C., and Baker, C. M. A. (1970). Molecular Biology and the Origin of Species University of Washington Press, Seattle, pp. 11–12.
Monahan, J. J., and Hall, R. H. (1974). Chromatin and gene regulation in eucaryotic cells at the transcriptional level. Crit. Rev. Biochem. 267.
Naso, R. B., Baker, F. T., Butcher, D., Kaczmarczyk, W., and Ulrich, V. (1975). Weight gain and heterosis at different stages of development of the rat. Growth 39345.
Naso, R. B., Baker, F. T., Kaczmarczyk, W., and Ulrich, V. (1976). Variation in rat liver chromatin composition during growth and the heterotic response. Biochem. Genet. 14281.
Panyim, S., and Chalkley, R. (1969a). High resolution acrylamide gel electrophoresis of histones. Arch. Biochem. Biochphys. 130337.
Panyim, S., and Chalkley, R. (1969b). A new histone found only in mammalian tissues with little cell division. Biochem. Biophys. Res. Comm. 371042.
Panyim, S., and Chalkley, R. (1969c). The heterogeneity of histones. A quantitative analysis of calf histones in very long polyacrylamide gels. Biochemistry 83972.
Pogo, B. G. T., Allfrey, V. G., and Mirsky, A. E. (1966). RNA synthesis and histone acetylation during the course of gene activation in lymphocytes. Proc. Natl. Acad. Sci. USA 55805.
Prestayko, A. W., and Busch, H. (1968). Low molecular weight RNA of the chromatin fraction from Novikoff hepatoma and rat liver nuclei. Biochim. Biophys. Acta 169327.
Pruitt, S. C., and Grainger, R. M. (1980). A repeating unit of higher order chromatin structure in chick red blood cell nuclei. Chromosoma 78257.
Ramponi, G., Nassi, P., Liguri, G., and Cappugi, G. (1978). Purification and properties of a histone-specific protease from rat liver chromatin. FEBS Lett. 90228.
Reeder, R. H. (1973). Transcription of chromatin by bacterial RNA polymerase. J. Mol. Biol. 80229.
Riggs, M. G., Whittaker, R. G., Neumann, J. R., and Ingram, V. M. (1977). n-Butyrate causes histone modification in HeLa and Friend erythroleukemia cells. Nature 268462.
Schneider, H. (1973). Methods for botanical sciences. In Clark, G. (ed.), Staining Procedures Williams and Wilkins, Baltimore, Md., pp. 226–227.
Sollner-Webb, B., Camerini-Otero, R. D., and Felsenfeld, G. (1976). Chromatin structure as probed by nucleases and proteases: Evidence for the central role of histones H3 and H4. Cell 9179.
Tallman, G. (1977). Dissertation, West Virginia University, Morgantown.
Tallman, G., Butcher, D., Kaczmarczyk, W., and Ulrich, V. (1977). In vitro transcriptional probes of heterotic rat liver chromatin. J. Hered. 68413.
Tallman, G., Kaczmarczyk, W., and Ulrich, V. (1978). Chromatographic comparisons of heterotic rat liver histones. J. Hered. 69282.
Tallman, G., Amero, S., Kaczmarczyk, W., and Ulrich, V. (1979). Electrophoretic comparisons of liver chromatin proteins isolated from heterotic rats during postweaned development. Biochem. Genet. 17185.
Walker, J. M., Goodwin, G. H., and Johns, E. W. (1978). The isolation and identification of ubiquitin from the high mobility group nonhistone protein fraction. FEBS Lett. 90327.
Webster, R. A., and Spelsberg, T. (1979). Steroid receptor binding to nuclei. J. Steroid Biochem. 10343.
Wilson, E. M., and Spelsberg, T. C. (1973). Rapid isolation of total acidic proteins from chromatin of various chick tissues. Biochim. Biophys. Acta 322145.
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Published with the approval of the Director of the West Virginia University Agricultural and Forestry Experiment Station as Scientific Paper No. 1616.
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Amero, S., Tallman, J.G., Kaczmarczyk, W. et al. Comparisons of liver chromatin proteins and template activities in parental and heterotic rats during postweaned development. Biochem Genet 21, 579–594 (1983). https://doi.org/10.1007/BF00484448
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DOI: https://doi.org/10.1007/BF00484448