Abstract
Erythrocyte peptidase-1 was analyzed by electrophoresis in various types of triploid Pleurodeles waltlii. Densitometric analysis of the zymograms showed (1) the existence of a gene-dose effect and (2) the presence of two Pep-1B alleles for one Pep-1A allele in heterozygous triploid females of biparental origin. Owing to the sex linkage of the PEP-1 locus (alleles Pep-1A and Pep-1B situated on the Z and W sex chromosomes, respectively), the results show that the sex genotype of these females is ZWW. In a particular line called series 103, the existence of a null allele was demonstrated. Densitometric analysis of females which were Pep-1A/Pep-10 (ZW), Pep-10/Pep-10 (WW), and Pep-1A/Pep-10/Pep-10 (ZWW) confirmed the gene-dose effect.
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This work was carried out under the programme of the Equipe de Recherche Associée No. 327 (CNRS) and partially supported by the University of Toulouse-Le Mirail and by a grant from the Centre National de la Recherche Scientifique (ATP “Biochimie du Développement,” 1980–1982).
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Cayrol, C., Jaylet, A., Ferrier, V. et al. A genetic study of various enzyme polymorphisms in Pleurodeles waltlii (Urodele, Amphibian). III. The relationship between sex-linked peptidase-1 expression and gene-dose effects. Biochem Genet 21, 551–559 (1983). https://doi.org/10.1007/BF00484446
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DOI: https://doi.org/10.1007/BF00484446