Abstract
The existence of four peptidases was demonstrated by starch gel electrophoresis in Pleurodeles waltlii: PEP-1, PEP-2, PEP-3, and PEP-4. Peptidases-3 and -4 are monomorphic, and peptidases-1 and -2 are polymorphic. The heredity of the polymorphisms was studied using individuals arising from crosses or of gynogenetic origin. Peptidase-1 is dimeric; its polymorphism depends on a pair of codominant alleles, Pep-1A and Pep-1B, which are situated on the Z and W sex chromosomes, respectively, in close proximity to, or even within, the sex differential segment. As the differential segment is very close to the centromere, the PEP-1 locus therefore also appears to be closely linked to it. Expression of the PEP-1 locus was shown to be independent of the sex hormone environment. This locus is the first case reported in amphibians of an enzyme marker linked to the genetic sex. It allows the sex of Pleurodeles to be determined before they reach sexual maturity. Peptidase-2 is monomeric. Its polymorphism depends on a pair of codominant alleles on an autosomal PEP-2 locus. The high proportion of heterozygous animals in the gynogenetic offspring of females heterozygous for the PEP-2 locus indicates segregation which is independent of the centromere. Analysis of the offspring of doubly heterozygous females (i.e., for two of the loci—LDH-B, G6PDH, PEP-1, and PEP-2) shows that the four loci are independent.
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References
Beckmann, L., and Johnson, F. M. (1964). Genetic control of aminopeptidases in Drosophila melanogaster. Hereditas 51221.
Collenot, A. (1973). Obtention, par la méthode des greffes de gonades embryonnaires, d'une femelle à descendance unisexuée femelle chez le Triton Pleurodeles waltlii Michah. Experientia 29885.
Dessauer, H. C., Nevo, E., and Chuang, K. C. (1975). High genetic variability in an ecologically variable vertebrate, Bufo viridis. Biochem. Genet. 13651.
Dikinson, W. J., and Sullivan, D. T. (1975). Gene-Enzyme Systems in Drosophila, Springer Verlag, Berlin.
Ferrier, V. (1981). La gynogenèse expérimentale et son application à l'étude de l'hérédité de divers polymorphismes enzymatiques dans le genre Pleurodeles (Amphibiens, Urodèles), Thèse Doctorat d'Etat 1013, Université Paul Sabatier, Toulouse, France.
Gallien, L. (1954). Inversion expérimentale du sexe sous l'action des hormones sexuelles chez le Triton Pleurodeles waltlii Michah. Analyse des conséquences génétiques. Bull. Biol. Fr. Belg. 11.
Gasser, F., Ferrier, V., Jaylet, A., and Deparis, P. (1983). A genetic study of various enzyme polymorphisms in Pleurodeles waltlii (Urodele Amphibian). I. Lactate dehydrogenase-B and glucose-6-phosphate dehydrogenase. Biochem. Genet. 21527.
Harris, H., and Hopkinson, D. A. (1977). Handbook of Enzyme Electrophoresis in Human Genetics Elsevier North-Holland, Amsterdam.
Highton, R., and Webster, T. P. (1976). Geographic protein variation and divergence in populations of the Salamander Plethodon cinereus. Evolution 3033.
Houillon, C., and Dournon, C. (1978). Inversion du phénotype sexuel femelle sous l'action d'une température élevée chez l'Amphibien Urodèle Pleurodeles waltlii Michah. C.R. Acad. Sci. Paris 2861475.
Human Gene Mapping 5 . (1979). Fifth International Workshop on Human Gene Mapping. Birth Effects: Original Article Series 15, No. 11, The National Foundation , New York .
Jaylet, A., and Ferrier, V. (1978). Experimental gynogenesis in the Newt Species Pleurodeles waltlii and P. poireti. Chromosoma (Berl.) 6965.
Jaylet, A., Ferrier, V., and Andrieux, B. (1980). Sur un facteur récessif apparu après mutagenèse aux rayons X affectant l'adaptation pigmentaire chez le Triton Pleurodeles waltlii. C.R. Acad. Sci. Paris 291673.
Lacroix, J. C. (1968). Etude descriptive des chromosomes en écouvillon dans le genre Pleurodeles (Amphibien, Urodèle). Ann. Embryol. Morphol. 1179.
Lewis, W. H. P., and Harris, H. (1967). Human red cell peptidases. Nature 215351.
Lewis, W. H. P., and Truslove, G. M. (1969). Electrophoretic heterogeneity of mouse erythrocyte peptidases. Biochem. Genet. 3493.
Lorand, L. (1976). Proteolytic enzymes. Methods Enzymol. XLV(B).
Moretti, J., Boussier, G., and Jayle, M. F. (1957). Réalisation technique et premières applications de l'électrophorèse sur gel d'amidon. Bull. Soc. Chim. Biol. 39593.
Nace, G. W., Richards, C. M., and Asher, J. H. (1970). Parthenogenesis and genetic variability. I. Linkage and imbreeding estimation in the frog, Rana pipiens. Genetics 66349.
Nagy, A., Rajki, K., Bakos, J., and Csnayi, V. (1979). Genetic analysis in carp (Cyprinus carpio) using gynogenesis. Heredity 4335.
Pasteur, N. (1975). Les leucine-aminopeptidases du Moustique Culex pipiens: génétique formelle d'un locus chez l'imago. C.R. Acad. Sci. Paris 280113.
Siciliano, M. J., and Shaw, C. R. (1976). In Smith, I. (ed.), Chromatographic and Electrophoretic Techniques William Heinemann, Books, London, p. 185.
Singh, R. S., Hubby, J. L., and Throckmorton, L. H. (1975). The study of genic variation by electrophoretic and heat denaturation techniques at the octarol dehydrogenase locus in markers of the Drosophila viridis group. Genetics 80637.
Szymura, J. M., and Farana, I. (1978). Inheritance and linkage analysis of five enzyme loci in interspecific hybrids of toadlets genus Bombina. Biochem. Genet. 16307.
Trippa, G., Catamo, A., Lombardozzi, A., and Cicchetti, K. (1978). A simple approach for discovering common non-electrophoretic enzym variability: A heat denaturation study in Drosophila melanogaster. Biochem. Genet. 16299.
Voelker, R. A., and Langley, C. H. (1978). Dipeptidase A: A polymorphic locus in Drosophila melanogaster. Genetica 49233.
Wright, D. A., Huang, C. P., and Choke, B. D. (1976). Meiotic origin of triploidy in the frog detected by genetic analysis of enzyme polymorphisms. Genetics 84319.
Yut, J., and Weitkamp, L. R. (1979). Equine peptidases: Correspondence with human peptidases and polymorphism for erythrocyte peptidase A. Biochem. Genet. 17987.
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This work was carried out under the programme of the Equipe de Recherche Associée No. 327 (CNRS) and partially supported by the University of Toulouse-Le Mirail and by a grant from the Centre National de la Recherche Scientifique (ATP “Biochimie du Developpement,” 1980–1982).
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Ferrier, V., Gasser, F., Jaylet, A. et al. A genetic study of various enzyme polymorphisms in Pleurodeles waltlii (Urodele Amphibian). II. Peptidases: Demonstration of sex linkage. Biochem Genet 21, 535–549 (1983). https://doi.org/10.1007/BF00484445
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DOI: https://doi.org/10.1007/BF00484445