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Identification of inherited protein variants in individual erythrocytes

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Abstract

Inherited electrophoretic variants of hemoglobin, carbonic anhydrase, and glucose-6-phosphate dehydrogenase in individual erythrocytes were separated by electrophoresis in ultrathin agar gels. By staining the electropherograms with specific fluorescein-conjugated antibodies against hemoglobins, relative proportions of two hemoglobins within individual erythrocytes can be estimated. The findings suggested that the intracellular proportions of HbA and HbS in heterozygotes are heterogeneous within a given population of cells. By this method cells containing hemoglobin F (F cells) as well as a minor variant of hemoglobin F were identified. This tool potentially offers an approach to monitoring distribution of inherited variants in individual erythrocytes for a large number of proteins.

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Authors and Affiliations

  1. Division of Medical Genetics, Department of Pediatrics and Child Health, and Center for Sickle Cell Disease, Howard University College of Medicine, 20059, Washington D.C.

    Stephen I. O. Anyaibe & Verle E. Headings

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  1. Stephen I. O. Anyaibe
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  2. Verle E. Headings
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This work was supported by a grant from the John A. Hartford Foundation and from the National Institutes of Health, Grant No. HL 15160.

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Anyaibe, S.I.O., Headings, V.E. Identification of inherited protein variants in individual erythrocytes. Biochem Genet 18, 455–463 (1980). https://doi.org/10.1007/BF00484394

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  • DOI: https://doi.org/10.1007/BF00484394

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