The arrangement of repetitive and nonrepetitive DNA sequences in the soybean genome was ascertained by a comparison of the reassociation kinetics of short (250 nucleotides) and long (2700 nucleotides) DNA fragments, the size distribution of S-1 nuclease resistant repetitive duplexes, and a direct assay of the spectrum of DNA sequences present on long DNA fragments enriched in repetitive DNA. These measurements reveal the following: (1) The 1N genome size of the soybean plant is 1.97 pg. (2) Approximately 40% of the soybean genome consists of nonrepetitive or single-copy DNA sequences, while 60% is repetitive DNA. (3) The repetitive DNA is partitioned into three discrete classes termed “very fast,” “fast,” and “slow,” containing DNA sequences repeated an average of 290,000, 2800, and 19 times each. (4) Approximately 35–50% of the soybean genome is arranged in a short-period interspersion pattern of 250 nucleotide slow sequences and single-copy DNA averaging up to 2700 nucleotides in length. (5) From 30% to 45% of the soybean genome is organized into long stretches of repetitive DNA at least 1500 nucleotides in length. (6) Minimal interspersion of repetitive sequence classes occurs in soybean DNA.
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Angerer, R. C., Davidson, E. H., and Britten, R. J. (1975). DNA sequence organization in the mollusc Aplysia californica. Cell 629.
Angerer, R. C., Davidson, E. H., and Britten, R. J. (1976). Single copy DNA and structural gene sequence relationships among four sea urchin species. Chromosoma 56213.
Bendich, A. J., and McCarthy, B. J. (1970). DNA comparisons among barley, oats, rye, and wheat. Genetics 65545.
Britten, R. J., and Davidson, E. H. (1969). Gene regulation in higher cells—A theory. Science 165349.
Britten, R. J., and Davidson, E. H. (1976). DNA sequence arrangement and preliminary evidence on its evolution. Fed. Proc. 352151.
Britten, R. J., and Smith, J. (1970). A bovine genome. Carnegie Inst. Wash. Year Book 68378.
Britten, R. J., Pavich, M., and Smith, J. (1970). A new method for DNA purification. Carnegie Inst. Wash. Year Book 68400.
Britten, R. J., Graham, D. E., and Neufeld, B. R. (1974). An analysis of repeating DNA sequences by reassociation. Methods Enzymol. 29E363.
Cech, T. R., and Hearst, J. E. (1976). Organization of highly-repeated sequences in mouse main-band DNA. J. Mol. Biol. 100227.
Chamberlin, M. E., Britten, R. J., and Davidson, E. H. (1975). Sequence organization in Xenopus DNA studied by the electron microscope. J. Mol. Biol. 96317.
Crain, W. R., Davidson, E. H., and Britten, R. J. (1976a). Contrasting patterns of DNA sequence arrangement in Apis mellifera (honeybee) and Musca domestica (housefly). Chromosoma 591.
Crain, W. R., Eden, F. C., Pearson, W. R., Davidson, E. H., and Britten, R. J. (1976b). Absence of short period interspersion of repetitive and non-repetitive sequences in the DNA of Drosophila melanogaster. Chromosoma 56309.
Davidson, E. H., and Britten, R. J. (1973). Organization, transcription, and regulation in the animal genome. Quart. Rev. Biol. 48555.
Davidson, E. H., Hough, B. R., Amenson, C. S., and Britten, R. J. (1973). General interspersion of repetitive with nonrepetitive elements in the DNA of Xenopus. J. Mol. Biol. 771.
Davidson, E. H., Graham, D. E., Neufeld, B. R., Chamberlin, M. E., Amenson, C. S., Hough, B. R., and Britten, R. J. (1974). Arrangement and characterization of repetitive sequence elements in animal DNAs. Cold Spring Harbor Symp. Quant. Biol. 38295.
Davidson, E. H., Galau, G. A., Angerer, R. C., and Britten, R. J. (1975). Comparative aspects of DNA sequence organization in metazoa. Chromosoma 51253.
Deininger, P. L., and Schmid, C. W. (1976). An electron microscope study of the DNA sequence organization of the human genome. J. Mol. Biol. 166773.
Firtel, R. A., and Kindle, K. (1975). Structural organization of the genome of the cellular slime mold Dictyostelium discoideum: Interspersion of repetitive and single copy DNA. Cell 5401.
Flavell, R. B., and Smith, D. B. (1976). Nucleotide sequence organization in the wheat genome. Heredity 37231.
Flavell, R. B., Bennett, M. D., Smith, J. B., and Smith, D. B. (1974). Genome size and proportion of repeated nucleotide sequence DNA in plants. Biochem. Genet. 4257.
Galau, G. A., Chamberlin, M. E., Hough, B. R., Britten, R. J., and Davidson, E. H. (1976). Evolution of repetitive and nonrepetitive DNA. In Ayala, F. (ed.), Molecular Evolution, Sinauer Assoc., Sunderland, Mass., pp. 200–224.
Goldberg, R. B., Bemis, W. P., and Siegel, A. (1972). Nucleic acid hybridization studies within the genus Cucurbita. Genetics 72253.
Goldberg, R. B., Crain, W. R., Ruderman, J. V., Moore, G. P., Barnett, T. R., Higgens, R. C., Gelfand, R. A., Britten, R. J., and Davidson, E. H. (1975). DNA sequence organization in the genomes of five marine invertebrates. Chromosoma 51225.
Manning, J. E., Schmid, C. W., and Davidson, N. (1975). Interspersion of repetitive and non-repetitive DNA sequences in the Drosophila melanogaster genome. Cell 4141.
Schmid, C. W., Manning, J. E., and Davidson, N. (1975) Inverted repeat sequences in the Drosophila genome. Cell 5159.
Smith, D. B., and Flavell, R. B. (1977). Nucleotide sequence organization in the rye genome. Biochim. Biophys. Acta 47482.
Smith, M. J., Britten, R. J., and Davidson, E. H. (1975). Studies on nucleic acid reassociation kinetics: Reactivity of single-stranded tails in DNA-DNA renaturation. Proc. Natl. Acad. Sci. 724805.
Studier, F. W. (1965). Sedimentation studies of the size and shape of DNA. J. Mol. Biol. 11373.
Thompson, W. F. (1976). Aggregate formation from short fragments of plant DNA. Plant Physiol. 57617.
Walbot, V., and Dure, L. S. (1976). Developmental biochemistry of cotton seed embryogenesis and germination. VII. Characterization of the cotton genome. J. Mol. Biol. 101 503.
Wells, R., Royer, H. D., and Hollenberg, C. P. (1976). Non Xenopus-like DNA sequence organization in the Chironomus tentons genome. Mol. Gen. Genet. 14745.
Wetmur, J. G., and Davidson, N. (1968). Kinetics of renaturation of DNA. J. Mol. Biol. 31349.
Zimmerman, J. L., and Goldberg, R. B. (1977). DNA sequence organization in the genome of Nicotiana tabacum. Chromosoma 59227.
These experiments were supported by NSF Grants BMS74-21461 and PCM76-24593 and were conducted while the author was in the Department of Biology, Wayne State University, Detroit, Michigan.
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Goldberg, R.B. DNA sequence organization in the soybean plant. Biochem Genet 16, 45–68 (1978). https://doi.org/10.1007/BF00484384