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Inheritance behavior of information coding for small subunit polypeptides of fraction 1 protein

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Abstract

In various genera of plants, the small subunit of fraction 1 protein is often composed of more than one kind of polypeptide; these differ in isoelectric points and amino acid composition. Previous analysis of numerous individual progeny of Nicotiana tabacum (two kinds of polypeptides), N. glauca + N. langsdorffii parasexual hybrids (three kinds), and other examples showed no change in F-1 protein composition as a consequence of alternation of generations. Experiments reported here show that absence of one member of each of the 24 different pairs of chromosomes in an N. tabacum monosomic series and also absence of the “S” pair in a nullisome did not affect F-1 protein composition. Absence of the “E” pair caused reduction in the amount of the least acidic of the two kinds of N. tabacum small subunit polypeptides. The question of how many individual progeny of self-fertile hybrids would have to be analyzed to detect segregation of genes coding for F-1 protein small subunit polypeptides, if segregation occurs, was answered by analysis of F1 hybrids between N. otophora and N. tomentosiformis, and two subspecies of N. suaveolens, together with their F2 progeny. In both cases, analysis of 16 progeny was sufficient to demonstrate a segregation pattern of two F1 hybrid type to one each of the two parental types. Therefore, in the absence of segregation, it is likely that coding information for different kinds of F-1 protein small subunit polypeptides is sequestered on heterologous chromosomes, as postulated in previous reports.

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Authors and Affiliations

  1. Department of Biology, Molecular Biology Institute, University of California, 90024, Los Angeles, California

    Kevin Chen & S. G. Wildman

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  1. Kevin Chen
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  2. S. G. Wildman
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Research supported by NSF Grant 75-07368 and contract no. EY-76-S-03-0034, P.A. #8 from the Department of Energy.

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Chen, K., Wildman, S.G. Inheritance behavior of information coding for small subunit polypeptides of fraction 1 protein. Biochem Genet 18, 1175–1184 (1980). https://doi.org/10.1007/BF00484346

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  • DOI: https://doi.org/10.1007/BF00484346

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