Biochemical Genetics

, Volume 16, Issue 5–6, pp 443–454 | Cite as

Genetic regulation of liver alcohol dehydrogenase in Peromyscus

  • Karen G. Burnett
  • Michael R. Felder
Article

Abstract

Data from genetic crosses of Peromyscus maniculatus and P. polionotus suggest that electrophoretic variants of liver alcohol dehydrogenase are coded by alleles at a single locus. These alleles, designated Adh F , Adh S , and Adh N , determine, respectively, the fast, slow, and not detectable (null) ADH electrophoretic phenotypes. Heterozygotes (Adh F /Adh S ) exhibit three bands on zymograms, suggesting a dimeric subunit structure for the enzyme. However, Adh F /Adh N and Adh S /Adh N animals exhibit a single band, suggesting that the Adh N allele does not produce a polypeptide subunit capable of dimerizing into an active molecule. Fast and slow electrophoretic phenotypes exhibit multiple bands which can be converted into single major fast and slow bands, respectively, upon treatment with oxidized or reduced NAD. Addition of NAD also stabilizes both the fast and slow enzyme to heat inactivation at 60 C for at least 30 min.

Key words

liver alcohol dehydrogenase Peromyscus genetic polymorphism 

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Copyright information

© Plenum Publishing Corporation 1978

Authors and Affiliations

  • Karen G. Burnett
    • 1
  • Michael R. Felder
    • 1
  1. 1.Department of BiologyUniversity of South CarolinaColumbia

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