Biochemical Genetics

, Volume 16, Issue 5–6, pp 415–432 | Cite as

Functional mutants of yeast alcohol dehydrogenase affecting kinetics, cellular redox balance, and electrophoretic mobility

  • Christopher Wills
  • Julia Phelps


Repeated selection of petite (respiratorily incompetent) Saccharomyces cerevisiae on medium containing allyl alcohol, both on plates and in the turbidostat, results in mutants with a remarkably similar response. Most of the mutations affect the constitutive alcohol dehydrogenase, resulting in enzymes with a cathodal shift in electrophoretic mobility, and none shows a significant anodal shift. The genetics, kinetics, and physiological effect of three of the mutants have been investigated in detail, and while all confer resistance to allyl alcohol through a shift in the NAD/NADH ratio, they do so in slightly different ways. The potential of this system for exploring the range of short-term adaptations open to this organism is discussed.

Key words

petite yeast allyl alcohol selection NAD/NADH ratio 


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Copyright information

© Plenum Publishing Corporation 1978

Authors and Affiliations

  • Christopher Wills
    • 1
  • Julia Phelps
    • 1
  1. 1.Department of BiologyUniversity of CaliforniaSan Diego

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