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Biochemical Genetics

, Volume 16, Issue 3–4, pp 307–319 | Cite as

Inheritance and linkage analysis of five enzyme loci in interspecific hybrids of toadlets, genus Bombina

  • Jacek M. Szymura
  • Irena Farana
Article

Abstract

Progeny produced from Bombina bombina, B. variegata, and field-collected interspecific hybrids have been analyzed for the inheritance of five enzyme loci, which are fixed for alternate alleles in the parental species. Lactate dehydrogenase (Ldh-1), NAD-dependent malate dehydrogenase (Mdh-1), creatine kinase (Ck), adenylate kinase (Ak), and glucosephosphate isomerase (Gpi) are all inherited in a Mendelian manner as codominant alleles at nuclear loci. Both parental alleles are equally functional in artificial F1 hybrids (female B. bombina×male B. variegata) at each of the loci studied. No linkage between any pair of loci was observed. Discovery of this inherited biochemical variation combined with a technique for assaying individual genotypes without killing the animals makes feasible studies of hybrid population structure heretofore impossible.

Key words

Bombina hybrids allozymes genetics 

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References

  1. Hunt, W. G., and Selander, R. K. (1973). Biochemical genetics of hybridization in European house mouse. Heredity 3111.Google Scholar
  2. Manwell, C., and Baker, C. A. M. (1970). Molecular Biology and the Origin of Species: Heterosis, Protein Polymorphism, and Animal Breeding, Sidgwick and Jackson, London, 394 pp.Google Scholar
  3. Morescalchi, A. (1965). Osservazioni sulla cariologia di Bombina. Boll. Zool. 32207.Google Scholar
  4. Pizzimenti, J. J. (1976). Genetic divergence and morphological convergence in the prairie dogs, Cynomys gunnisoni and Cynomys leucurus. II. Genetic analysis. Evolution 30367.Google Scholar
  5. Shaw, C. R., and Prasad, R. (1970). Starch gel electrophoresis of enzymes—A compilation of recipes. Biochem. Genet. 4297.Google Scholar
  6. Szymura, J. M. (1976a). Hybridization between discoglossid toads Bombina bombina and Bombina variegata in southern Poland as revealed by the electrophoretic technique. Z. Zool. Syst. Evol.-Forsch. 14227.Google Scholar
  7. Symura, J. M. (1976a). New data on the hybrid zone between Bombina bombina and Bombina variegata (Anura, Discoglossidae). Bull. Acad. Polon. Sci. Cl. II 24355.Google Scholar
  8. Tunner, H. G. (1973). Das Albumin und andere Bluteiweisse bei Rana ridibunda Pallas, Rana lessonae Camerano, Rana esculenta Linne und deren Hybriden. Z. Zool. Syst. Evol.-Forsch. 11219.Google Scholar
  9. Uzzell, T., and Berger, L. (1975). Electrophoretic phenotypes of Rana ridibunda, Rana lessonae, and their hybridogenetic associate, Rana esculenta. Proc. Natl. Acad. Sci. 12713.Google Scholar
  10. Uzzell, T., Berger, L., and Günther, R. (1975). Diploid and triploid progeny from a diploid female of Rana esculenta (Amphibia, Salientia). Proc. Natl. Acad. Sci. 12781.Google Scholar
  11. Vogel, P., and Chen, P. S. (1976). Genetic control of LDH isozymes in the Rana esculenta complex. Experientia 32304.Google Scholar
  12. Whitt, G. S., Cho, P. L., and Childers, W. F. (1972). Preferential inhibition of allelic isozyme synthesis in an interspecific sunfish hybrid. J. Exp. Zool. 179271.Google Scholar
  13. Wright, D. A. (1973). Inheritance of twelve enzyme loci in hybrids of Rana pipiens pipiens and R. P. berlandieri. Am. Zool. 131320 (abst.).Google Scholar

Copyright information

© Plenum Publishing Corporation 1978

Authors and Affiliations

  • Jacek M. Szymura
    • 1
  • Irena Farana
    • 1
  1. 1.Department of Comparative AnatomyJagellonian UniversityKrakówPoland

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