Biochemical Genetics

, Volume 16, Issue 3–4, pp 191–202 | Cite as

Expression of lactate dehydrogenase isozyme 5 (LDH-5) in cultured mouse blastocysts in the absence of implantation and outgrowth

  • Horst Spielmann
  • Hans-Georg Eibs
  • Ursula Jacob-Müller
  • Rosemarie Bischoff


Extensive extraction studies with Triton X-100 revealed only LDH-1 (B4) but no trace of LDH-5 (A4) in one-cell and two-cell mouse and rat embryos. The LDH isozyme pattern of preimplantation mouse embryos changes from the maternally inherited B subunit isozyme (LDH-1) to a pattern dominated by LDH-5 when mouse blastocysts are cultured under conditions that prevent hatching but allow trophoblast giant cell transformation. During differentiation of mouse blastocysts in vitro, implantation is therefore not essential for the appearance of the A subunit form of LDH (LDH-5) coded for by the embryonic genome. Mechanisms controlling the expression of LDH-5 in mouse blastocysts during in vivo development are discussed.

Key words

lactate dehydrogenase isozymes rat and mouse ova mouse blastocyst implantation Triton X-100 


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Copyright information

© Plenum Publishing Corporation 1978

Authors and Affiliations

  • Horst Spielmann
    • 1
  • Hans-Georg Eibs
    • 1
  • Ursula Jacob-Müller
    • 1
  • Rosemarie Bischoff
    • 1
  1. 1.Institut für Toxikologie und Embryonal-Pharmakologie der Freien Universität BerlinBerlin 33West Germany

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