Biochemical Genetics

, Volume 16, Issue 3–4, pp 191–202 | Cite as

Expression of lactate dehydrogenase isozyme 5 (LDH-5) in cultured mouse blastocysts in the absence of implantation and outgrowth

  • Horst Spielmann
  • Hans-Georg Eibs
  • Ursula Jacob-Müller
  • Rosemarie Bischoff
Article

Abstract

Extensive extraction studies with Triton X-100 revealed only LDH-1 (B4) but no trace of LDH-5 (A4) in one-cell and two-cell mouse and rat embryos. The LDH isozyme pattern of preimplantation mouse embryos changes from the maternally inherited B subunit isozyme (LDH-1) to a pattern dominated by LDH-5 when mouse blastocysts are cultured under conditions that prevent hatching but allow trophoblast giant cell transformation. During differentiation of mouse blastocysts in vitro, implantation is therefore not essential for the appearance of the A subunit form of LDH (LDH-5) coded for by the embryonic genome. Mechanisms controlling the expression of LDH-5 in mouse blastocysts during in vivo development are discussed.

Key words

lactate dehydrogenase isozymes rat and mouse ova mouse blastocyst implantation Triton X-100 

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References

  1. Auerbach, S., and Brinster, R. L. (1967). Lactate dehydrogenase isozymes in the early mouse embryo. Exp. Cell Res. 4689.Google Scholar
  2. Auerbach, S., and Brinster, R. L. (1968). Lactate dehydrogenase isozymes in mouse blastocyst cultures. Exp. Cell Res. 53313.Google Scholar
  3. Barlow, P. W., and Sherman, M. I. (1972). The biochemistry of differentiation of mouse trophoblast: Studies on polyploidy. J. Embryol. Exp. Morphol. 27447.Google Scholar
  4. Besley, G. T. N. (1976). Effect of Triton X-100 on the isoelectric focusing profile of fibroblast sphingomyelinase. FEBS Lett. 72101.Google Scholar
  5. Brinster, R. L. (1971). The lactate dehydrogenase in the preimplantation embryos of Quackenbush and Swiss mice. FEBS Lett. 1741.Google Scholar
  6. Engel, W., and Petzoldt, U. (1973). Early developmental changes of the lactate dehydrogenase isoenzyme pattern in mouse, rat, guinea-pig, Syrian hamster and rabbit. Humangenetik 20125.Google Scholar
  7. Epstein, C. J. (1975). Gene expression and macromolecular synthesis during preimplantation embryonic development. Biol. Reprod. 1282.Google Scholar
  8. Epstein, C. J., Kwok, L., and Smith, S. (1971). The source of lactate dehydrogenase in preimplantation mouse embryos. FEBS Lett. 1345.Google Scholar
  9. Erickson, R. P., Spielmann, H., Mangia, F., Tennenbaum, D., and Epstein, C. J. (1975). Studies on lactate dehydrogenase isozymes in gametes and early development of mice. In Markert, C. L. (ed.), Isozymes, Vol. III, Academic Press, New York, pp. 313–324.Google Scholar
  10. Fries, E. (1976). Determination of Triton X-100 binding to membrane proteins by polyacrylamide gel electrophoresis. Biochim. Biophys. Acta 455928.Google Scholar
  11. Mangia, F., Erickson, R. P., and Epstein, C. J. (1976). Synthesis of LDH-1 during mammalian oogenesis and early development. Dev. Biol. 54146.Google Scholar
  12. McLaren, A., and Hensleigh, H. C. (1975). Culture of mammalian embryos over the implantation period. In Balls, M., and Wild, A. E. (eds.), The Early Development of Mammals, Cambridge University Press, Cambridge, pp. 45–60.Google Scholar
  13. Monk, M., and Ansell, J. (1976). Patterns of lactic dehydrogenase isozymes in mouse embryos over the implantation period in vivo and in vitro. J. Embryol. Exp. Morphol. 36653.Google Scholar
  14. Monk, M., and Petzoldt, U. (1977). Control of inner cell mass development in cultured mouse blastocysts. Nature 265338.Google Scholar
  15. Peacock, T. A. R., Schultz, G. A., and Browder, L. W. (1976). Lactate dehydrogenase in one-cell rabbit embryos extracted with Triton X-100. Biochem. Genet. 14523.Google Scholar
  16. Pienkowski, M., Solter, D., and Koprowski, H. (1974). Early mouse embryos: Growth and differentiation in vitro. Exp. Cell Res. 85424.Google Scholar
  17. Poznakhirkina, N. A., Serov, O. L., and Korochkin, L. I. (1975). A study on lactate dehydrogenase isozymes in rat ova. Biochem. Genet. 1365.Google Scholar
  18. Rapola, J., and Koskimies, O. (1967). Embryonic enzyme patterns: Characterization of the single lactate dehydrogenase isozyme in preimplanted mouse ova. Science 1571311.Google Scholar
  19. Sherman, M. I. (1972a). Biochemistry of differentiation of mouse trophoblast: Esterase. Exp. Cell Res. 75449.Google Scholar
  20. Sherman, M. I. (1972b). The biochemistry of differentiation of mouse trophoblast: Alkaline phosphatase. Dev. Biol. 27337.Google Scholar
  21. Sherman, M. I. (1975). Long term culture of cells derived from mouse blastocysts. Differentiation 351.Google Scholar
  22. Spielmann, H., Erickson, R. P., and Epstein, C. J. (1973). The separation of lactate dehydrogenase X from other lactate dehydrogenase isozymes of mouse testes by affinity chromatography. FEBS Lett. 3519.Google Scholar
  23. Spielmann, H., Erickson, R. P., and Epstein, C. J. (1974a). Immunochemical studies of lactate dehydrogenase and glucose-6-phosphate dehydrogenase in preimplantation mouse embryos. J. Reprod. Fertil. 40367.Google Scholar
  24. Spielmann, H., Erickson, R. P., and Epstein, C. J. (1974b). The production of antibodies against mammalian LDH-1. Anal. Biochem. 59462.Google Scholar
  25. Spielmann, H., Eibs, H.-G., Mentzel, C., and Nagel, D. (1977). Studies on the binding of antibody against mouse lactate dehydrogenase (isoenzyme X) by preimplantation mouse embryos. J. Reprod. Fertil. 5047.Google Scholar
  26. Spindle, A. I., and Pedersen, R. A. (1973). Hatching, attachment, and outgrowth of mouse blastocysts in vitro: Fixed nitrogen requirements. J. Exp. Zool. 186305.Google Scholar
  27. Whitten, W. K. (1971). Nutrient requirements for the culture of preimplantation embryos in vitro. In Raspé, G. (ed.), Advances in the Biosciences, Vol. 6, Pergamon Press, New York, pp. 129–139.Google Scholar

Copyright information

© Plenum Publishing Corporation 1978

Authors and Affiliations

  • Horst Spielmann
    • 1
  • Hans-Georg Eibs
    • 1
  • Ursula Jacob-Müller
    • 1
  • Rosemarie Bischoff
    • 1
  1. 1.Institut für Toxikologie und Embryonal-Pharmakologie der Freien Universität BerlinBerlin 33West Germany

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