Abstract
In the F1 hybrids between Durrant's L and Durrant's S flax genotrophs, the relative mobilities of the anionic peroxidase isozymes were essentially the same as those in the L parent. The isozymes in both parents and their F1's were compared over a range of acrylamide gel concentrations, with plots of log relative mobility against gel concentration. Plots of comparative mobility, relative to the internal standard hemoglobin, against concentration were also examined. Both approaches provided evidence that apparent molecular weight modifications underlay the shift in mobility between the parents and the resemblance of the F1's to L, the parent which was homozygous for the dominant alleles controlling the mobility shift for at least two of the isozymes.
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References
Davis, B. J. (1964). Disc electrophoresis. II. Method and application to human serum proteins. Ann. N.Y. Acad. Sci. 121404.
Durrant, A. (1971). Induction and growth of flax genotrophs. Heredity 27277.
Ferguson, K. A. (1964). Starch-gel electrophoresis—application to the classification of pituitary proteins and polypeptides. Metabolism 13(10):985.
Fieldes, M. A., and Tyson, H. (1972). Activity and relative mobility of peroxidase isozymes in genotrophs and genotypes of flax (Linum usitatisimum L.). Can. J. Genet. Cytol. 14625.
Fieldes, M. A., and Tyson, H. (1973). Activity and relative mobility of peroxidase and esterase isozymes of flax (Linum usitatissimum) genotrophs. II. F1 hybrids and nuclear DNA reversion types. Can. J. Genet. Cytol. 15745.
Fieldes, M. A., and Tyson, H. (1981). Isozyme comparisons using weighted analyses of electrophoretic mobility in a range of polyacrylamide gel concentrations. Electrophoresis 2296.
Fieldes, M. A., Deal, C. L., and Tyson, H. (1977). Preliminary characterization of peroxidase isozymes isolated from two flax genotrophs. Can. J. Bot. 55(1):1465.
Gonenne, A., and Lebowitz, J. (1975). Estimation of molecular weights of small proteins on polyacrylamide gel electrophoresis. Anal. Biochem. 64414.
Johnson, G. B. (1977). Characterization of electrophoretically cryptic variation in the alpine butterfly Colias meadii. Biochem. Genet. 15665.
Rodbard, D., and Chrambach, A. (1974). Quantitative polyacrylamide gel electrophoresis: Mathematical and statistical analysis of data. Allen, R. C., and Maurer, H. R. (eds.), Electrophoresis and Isoelectric Focusing in Polyacrylamide Gel de Gruyter, Berlin, pp. 28–62.
Tyson, H., Taylor, S. A., and Fieldes, M. A. (1978). Segregation of the environmentally induced relative mobility shifts in flax genotroph peroxidase isozymes. Heredity 40(2):281.
Welinder, K. G. (1976). Covalent structure of the glycoprotein horseradish peroxidase (EC 1.11.1.7). FEBS Lett. 7219.
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The award of an operating grant from the National Research Council of Canada is acknowledged with thanks.
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Tyson, H., Fieldes, M.A. Molecular weight and net charge of peroxidase isozymes in F1 hybrids between L and S flax genotrophs. Biochem Genet 20, 919–927 (1982). https://doi.org/10.1007/BF00484069
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DOI: https://doi.org/10.1007/BF00484069