Abstract
The specific activity of creatine phosphokinase (CPK) was measured in the muscle of mdg/mdg and control embryos of 14–18 days' gestation. CPK specific activity values were similar in mutant and normal embryos at the earliest stages examined (14–15 days). However, after 15 1/2 days, the specific activity of the enzyme in the mdg/mdg embryos was approximately 50% lower than in the controls. The dysgenic and normal muscle extracts exhibited comparable stability after storage at −85 C. CPK activity levels in the muscle of adult heterozygotes (+/mdg) and wild-type (+/+) controls were found to be statistically identical. The findings suggest that the mdg mutation does not have a primary or direct effect on CPK activity.
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This research was supported by grants from the Charles and Johanna Busch Memorial Fund and Research Council of Rutgers University and by a grant from the Muscular Dystrophy Association of New York.
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Essien, F.B., Biber, C.L. Creatine phosphokinase activity in dysgenic (mdg/mdg) mouse muscle. Biochem Genet 15, 963–970 (1977). https://doi.org/10.1007/BF00483991
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DOI: https://doi.org/10.1007/BF00483991