Abstract
Formal genetic studies of sunflower (Helianthus annuus) seed acid phosphatase (ACP, E.C. 3.1.3.2) had suggested that the functional enzyme consists of two polypeptide subunits. The dimeric quaternary structure was demonstrated by dissociation-recombination procedures. Dissociation of electrophoretically distinct homodimers was effected upon freezing of extracts in a pH 8–9 buffer containing 1 m NaCl and 0.1 m 2-mercaptoethanol. Reassociation, as indicated by the formation of the hybrid isozyme, occurred during 12 hr dialysis against a pH 7.0 buffer.
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Supported by the University of Kansas General Research Fund Grant 3652.
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Torres, A.M., Diedenhofen, U. Dissociation-recombination of sunflower seed acid phosphatase. Biochem Genet 15, 897–901 (1977). https://doi.org/10.1007/BF00483985
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DOI: https://doi.org/10.1007/BF00483985