Biochemical Genetics

, Volume 15, Issue 9–10, pp 825–832 | Cite as

Translation of endogenous message in embryonic chick erythroid cell polysomes in a cell-free protein-synthesizing system

  • Nicholas Zagris
Article

Abstract

Polysomes from 5.5-day and 6.5-day embryonic chick erythroid cells contain messenger RNA (mRNA) which can be translated into products in a cell-free protein-synthesizing system. The products of the cell-free system coelectrophoresed with carrier globin chains from the cells from which the polysomal pellet was isolated. Wheat germ S30 fraction increases by 1.5–2.0 fold [3H] leucine incorporation into trichloroacetic acid-precipitable material directed by the endogenous mRNA on chick erythroid cell polysomes. The wheat germ probably provides a specific factor or factors absent or in shortage in the incubation medium.

Key words

chick embryo erythroid-cell polysomes cell-free system protein synthesis 

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Copyright information

© Plenum Publishing Corporation 1977

Authors and Affiliations

  • Nicholas Zagris
    • 1
  1. 1.The Biological LaboratoriesHarvard UniversityCambridge

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