Abstract
β-Hydroxy acid dehydrogenase (β-Had-2) of Anopheles albimanus was assigned to chromosome 3. The apparent sequence of loci on chromosome 3 is hexokinase-1-22-stripe-28-β-hydroxy acid dehydrogenase-2-4-aldehyde oxidase-2-esterase-8-4-esterase-4-?-phosphoglucomutase-?-esterase-6. β-Hydroxy acid dehydrogenase is 25 and 30 map units from phosphoglucomutase and esterase-6, respectively. The one-band electromorph of β-Had-2 in homozygotes and the three-band type in heterozygotes suggest that the enzyme is a dimer. A variety of electrophoretic techniques and spectrophotometric analysis were used to determine if the allozymes of β-Had-2 can be differentiated on a basis other than mobility. No differences were detected among the allozymes on the basis of thermostability, urea denaturation, response to thiol reagents, chelating agents, or changes in coenzyme and substrate concentrations. No heterogeneity within allozymes separated by electrophoresis was detected by using thermostability tests.
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References
Borak, L. I. (1974). Organ distribution of Drosophila β-hydroxy acid: NAD oxidase. Experientia 3031.
Borak, L. I., and Sofer, W. (1971). Drosophila β-L-hydroxy acid dehydrogenase. Purification and properties. J. Biol. Chem. 2465345.
Klebe, R. J. (1975). A simple method for the quantitation of isozyme patterns. Biochem. Genet. 13805.
Mahler, H. R., and Cordes, E. H. (1966). Biological Chemistry Harper and Row, New York.
McDowell, R. E., and Prakash, S. (1976). Allelic heterogeneity within allozymes separated by electrophoresis in Drosophila pseudoobscura. Proc. Natl. Acad. Sci. USA 734150.
Narang, S., Seawright, J. A., and Kitzmiller, J. B. (1981a). Linkage relationship and assignment of Esterase-4 and Esterase-8 loci to chromosome 3 in Anopheles albimanus Wiedeman. J. Hered. 72157.
Narang, S., Seawright, J. A., and Joslyn, D. (1981b). Inheritance and mapping of hexokinase-1 and phosphoglucomutase-1 in Anopheles albimanus. Mosq. News 4199.
Singh, R. S., Lewontin, R. C., and Felton, A. (1976). Genetic heterogeneity within electrophoretic “alleles” of xanthine dehydrogenase in Drosophila pseudoobscura. Genetics 84609.
Steiner, W. M. W., and Joslyn, D. (1979). Electrophoretic techniques for the genetic study of mosquitoes. Mosq. News 3935.
Tobler, J. E., and Grell, H. (1978). Genetics and physiological expression of β-hydroxy acid dehydrogenase in Drosophila. Biochem. Genet. 16333.
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Narang, S., Seawright, J.A. Genetic and physiochemical studies on β-hydroxy acid dehydrogenase in Anopheles albimanus . Biochem Genet 21, 885–893 (1983). https://doi.org/10.1007/BF00483947
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DOI: https://doi.org/10.1007/BF00483947