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Demonstration of 6-phosphogluconolactonase activity in Drosophila melanogaster using a null allele of 6-phosphogluconate dehydrogenase

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Abstract

Using a double mutant strain, Pgd n Zw n, we have developed an assay for 6-phosphogluconolactonase activity and have demonstrated its occurrence in adult Drosophila melanogaster.

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References

  • Brodie, A. F., and Lipmann, F. (1955). Identification of a gluconolactonase. J. Biol. Chem. 212677.

    Google Scholar 

  • Cori, O., and Lipmann, F. (1952). The primary oxidation product of enzymatic glucose-6-phosphate oxidation. J. Biol. Chem. 194417.

    Google Scholar 

  • Hughes, M. B., and Lucchesi, J. C. (1977). Genetic rescue of a lethal “null” activity allele of 6-phosphogluconate dehydrogenase in Drosophila melanogaster. Science 1961114.

    Google Scholar 

  • Kawada, M., Kagawa, Y., Takiguo, H., and Shimazono, N. (1962a). Purification of 6-phosphogluconolactonase from rat liver and yeast; its separation from gluconolactonase. Biochim. Biophys. Acta 57404.

    Google Scholar 

  • Kawada, M., Takiguchi, H., Kagawa, Y., Suzuki, K., and Shimazono, N. (1962b). Comparative studies on soluble lactonases. J. Biochem. 51405.

    Google Scholar 

  • Kupor, S. R., and Fraenkel, D. G. (1969). 6-Phosphogluconolactonase mutants of Escherichia coli and a maltose blue gene. J. Bacteriol. 1001296.

    Google Scholar 

  • Kupor, S. R., and Fraenkel, D. G. (1972). Glucose metabolism in 6-phosphogluconolactonase mutants of Escherichia coli. J. Biol. Chem. 2471904.

    Google Scholar 

  • Lipmann, F. (1951). The primary product of glucose-6-phosphate oxidation. In McElroy, W. D., and Glass, B. (eds.), Phosphorus Metabolism: A Symposium. Vol. 1, Johns Hopkins Press, Baltimore, pp. 158–160.

    Google Scholar 

  • Schofield, P. J., and Sols, A. (1976). Rat liver 6-phosphogluconolactonase: A low K m enzyme. Biochem. Biophys. Res. Commun. 711313.

    Google Scholar 

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Authors and Affiliations

  1. Genetics Curriculum and Department of Zoology, University of North Carolina, 27514, Chapel Hill, North Carolina

    M. Beatrice Hughes & John C. Lucchesi

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  1. M. Beatrice Hughes
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  2. John C. Lucchesi
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Additional information

This investigation was supported by Research Grant GM-15691 and Training Grant T01-GM-0685 of the National Institutes of Health.

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Hughes, M.B., Lucchesi, J.C. Demonstration of 6-phosphogluconolactonase activity in Drosophila melanogaster using a null allele of 6-phosphogluconate dehydrogenase. Biochem Genet 16, 1023–1029 (1978). https://doi.org/10.1007/BF00483752

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  • DOI: https://doi.org/10.1007/BF00483752

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