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Histochemistry

, Volume 84, Issue 4–6, pp 471–483 | Cite as

Immunocytochemical identification and localization of peptide hormones in the gastro-entero-pancreatic (GEP) endocrine system of the mouse and a stomachless fish, Barbus conchonius

  • I. H. W. M. Rombout
  • C. P. M. van der Grinten
  • F. M. Peeze Binkhorst
  • J. J. Taverne-Thiele
  • H. Schooneveld
Original Articles

Summary

A large number of antisera mainly raised against mammalian hormones are tested immunocytochemically on the GEP-endocrine system of mouse and fish (Barbus conchonius). The endocrine pancreas of mouse and fish appeared to contain the same four endocrine cell types; insulin-, glucagon-, PP- and somatostatin-immunoreactive cells.

In mouse about 13 GEP endocrine cell types are distinguished 1. insulin-, 2. somatostatin-, 3. glucagon-, 4. PP-, 5. (entero)glucagon-/PP-like, 6. CCK-like, 7. substance P-, 8. neurotensin-, 9. VIP-, 10. gastrin-, 11. secretin-, 12. β-endorphin-, 13. serotonin-immunoreactive cells.

Based on this and a previous study at least 13 GEP endocrine cell types seems to be present in stomachless fish: 1–9 as described for mouse, 10. (entero)glucagon-like, 11. met-enkephalin, 12. VIP-like, 13. unspecific immunoreactive endocrine cells.

Coexistence of glucagon and PP-like peptides is found in the gut and pancreas of mice and in the gut of B. conchonlus. In mouse pancreas and fish gut, endocrine cells showing only PP-or glucagon-like immunoreactivity are found too. In mouse stomach some endocrine cells, showing only PP-immunoreactivity are demonstrated. In the same region coexistence of C-1-gastrin-and FMRF-amide-immunoreactivity is found in endocrine cells. The importance of these phenomena are discussed.

Enteric nerves immunoreactive with antisera raised against substance P and GRP are found in mouse, against somatostatin and met-enkephalin in both mouse and fish and against VIP in fish.

Keywords

Peptide Glucagon Secretin Endocrine Cell Peptide Hormone 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • I. H. W. M. Rombout
    • 1
  • C. P. M. van der Grinten
    • 1
  • F. M. Peeze Binkhorst
    • 1
  • J. J. Taverne-Thiele
    • 1
  • H. Schooneveld
    • 2
  1. 1.Department of Experimental Animal Morphology and Cell BiologyAgricultural UniversityWageningenThe Netherlands
  2. 2.Department of EntomologyAgricultural UniversityWageningenThe Netherlands

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