Summary
Two-dimensional-electrophoretic analysis has been applied to non-neoplastic mammary epithelium from eight healthy women, tumor tissue from eight “diploid” mammary adenocarcinomas, and tumor tissue from eight “aneuploid” mammary adenocarcinomas. Compared with non-neoplastic mammary epithelium, a slight numerical net increase of the protein spots was detected in “diploid” tumors and a marked increase in “aneuploid” tumors. Two prominent spots were present in all 16 malignant tissues examined and absent in all eight non-neoplastic tissues (silver staining method).
The results suggest that a difference in the composition of cellular proteins exists both between non-neoplastic mammary cells and malignant tumor cells, and between “diploid” and “aneuploid” tumors.
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References
Auer G, Caspersson T, Wallgren A (1980) DNA content and survival in mammary carcinoma. Anal Quant Cytol 3:161
Auer G, Ono J, Caspersson T (1983) Determination of the fraction of Go cells in cytologic samples by means of simultaneous DNA and nuclear protein analyses. Anal Quant Cytol 5:5–8
Auer G, Ono J, Caspersson T (1983) Cytochemical identification of quiescent and growth activated tumor cells. Anal Quant Cytol 5:5–8
Auer G, Eriksson E, Azavedo E, Caspersson T, Wallgren A (1984) Prognostic significance of nuclear DNA content in mammary adenocarcinoma in humans. Cancer Res 44:394
Caspersson T, Lomakka G (1970) Recent progress in quantitative cytochemistry instrumentation and results. In: Wied GL, Bahr GF (eds) Introduction to quantitative cytochemistry II. Academic Press, New York, pp 27–56
Gaub J, Auer G, Zetterberg A (1975) Quantitative cytochemical aspects of a combined feulgen naphtol yellow S staining procedure for the simultaneous determination of nuclear and cytoplasmic protein and DNA in mammalian cells. Exp Cell Res 92:323
Klose J (1975) Protein mapping be combined isoelectric focusing and electrophoresis of mouse tissues. A novel approach to testing for induced point mutations in mammals. Humangenetik 26:231–243
Westerbrink K (1984) High resolution two-dimensional protein mapping. Thesis Groningen
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In honour of Prof. P. von Duijn
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Steinbeck, R., Steinbeck, C., Postel, E. et al. High-resolution 2-dimensional protein mapping of “diploid” and “aneuploid” mammary adenocarcinomas. Histochemistry 84, 338–341 (1986). https://doi.org/10.1007/BF00482960
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DOI: https://doi.org/10.1007/BF00482960