Summary
Lead is prevalently replaced by cerium as trapping agent in phosphatase cytochemistry to prevent nonspecific precipitation. Recently, substrate specific but artefactual lcad precipitates have been described in the nuclear envelope (NE) and rough endoplasmic reticulum (RER) due to a local matrix effect. In the present study a verification was carried out of the localization of acid phosphatase and glucose-6-phosphatase in the NE and RER of rat peritoneal macrophages and hepatocytes respectively with cerium. It appeared that precipitates of cerium phosphate in NE and RER of peritoneal macrophages do not represent sites of acid phosphatase activity but are due to the matrix effect. However, in rat hepatocytes these organelles demonstrate true reactive sites for glucose-6-phosphatase.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
De Jong ASH (1982) Mechanisms of metal-salt methods in enzyme cytochemistry with special reference to acid phosphatase. A review. Histochem J 14:1–33
De Jong ASH, Hak TJ, Duijn P van, Daems WTh (1979a) A new dynamic model system for the study of capture reactions for diffusable compounds in cytochemistry. II. Effect of the composition of the incubation medium on the trapping of phosphate ions in acid phosphatase cytochemistry. Histochem J 11:145–161
De Jong ASH, Hak TJ, Duijn P van, Daems WTh (1979b) A new dynamic model system for the study of capture reactions for diffusable compounds in cytochemistry. III. Influence of the matrix composition on the lead phosphate precipitation process in acid phosphatase cytochemistry. Histochem J 11:163–171
Hoefsmit ECM, Eestermans IL, Korn C, Duijn P van (1985) False localization of acid phosphatase activity in the nuclear envelope and endoplasmic reticulum of peritoneal macrophages. Histochem J 17:235–241
Hulstaert CE, Kalicharan D, Hardonk MJ (1983) Cytochemical demonstration of phosphatases in the rat liver by a ceriumbased method in combination with osmium tetroxide and potassium ferrocyanide posfixation. Histochemistry 78:71–79
Kalicharan D, Hulstaert CE, Hardonk MJ (1985) Prevention of penetration hindrance in cerium-based glucose-6-phosphate cytochemistry by freezing tissue in melting nitrogen. Histochemistry 82:287–292
Leskes A, Siekevitz P, Palade GE (1971a) Differentiation of endoplasmic reticulum in hepatocytes. I. Glucose-6-phosphatase distribution in situ. J Cell Biol 49:264–287
Leskes A, Siekevitz P, Palade GE (1971b) Differentiation of endoplasmic reticulum in hepatocytes. II. Glucose-6-phosphatase in rough microsomes. J Cell Biol 49:288–302
Robinson JM, Karnovsky MJ (1983) Ultrastructural localization of several phosphatases with cerium. J Histochem Cytochem 31:1197–1208
Teutsch HF (1978) Improved method for the histochemical demonstration of glucose-6-phosphatase activity. Histochemistry 57:107–117
Tice L, Barnett R (1962) The fine structural localization of glucose-6-phosphatase in rat liver. J Histochem Cytochem 10:754–762
Veenhuis M, Dijken JP van, Harder W (1980) A new method for the cytochemical demonstration of phosphatase activities in yeasts based on the use of cerous ions. FEMS Microbiol Lett 9:285–291
Wachstein M, Meisel E (1956) On the histochemical demonstration of glucose-6-phosphatase. J Histochem Cytochem 4:592
Author information
Authors and Affiliations
Additional information
In honour of Professor van Duijn
Rights and permissions
About this article
Cite this article
Hoefsmit, E.C.M., Hulstaert, C.E., Kalicharan, D. et al. Phosphatase cytochemistry with cerium as trapping agent. Histochemistry 84, 329–332 (1986). https://doi.org/10.1007/BF00482958
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00482958