Histochemistry

, Volume 84, Issue 4–6, pp 323–328 | Cite as

Fourier analysis of the cell shape of paired human urothelial cell lines of the same origin but of different grades of transformation

  • K. Ostrowski
  • A. Dziedzic-Goclawska
  • P. Strojny
  • W. Grzesik
  • J. Kieler
  • B. Christensen
  • M. Mareel
Original Articles

Summary

The rationale of the present investigation is the observations made by many authors of changes in the molecular structure of the cell surface during the multistep process of malignant transformation. These changes may influence cell-matrix and cell-cell interactions and thereby cause changes in cell adhesiveness and cell shape.

The aim of the present work was to investigate whether the development of various grades of transformation in vivo and in vitro of human urothelial cells is accompanied by significant changes in cell shape as measured by Fourier analysis.

The following transformation grades (TGr) have been defined (Christensen et al. 1984; Kieler 1984): TGr I=nonmalignant, mortal cell lines that grow independently of fibroblasts and have a prolonged life span. TGr II=nonmalignant cell lines with an infinite life span. TGr III=malignant and immortal cell lines that grow invasively in co-cultures with embryonic chick heart fragments and possess tumorigenic properties after s.c. injection into nude mice.

Comparisons of 4 pairs of cell lines were performed; each pair was of the same origin. Two pairs-each including a TGr I cell line (Hu 961b and Hu 1703S) compared to a TGr III cell line (Hu 961a or Hu 1703He)-were derived from two transitional cell carcinomas (TCC) containing a heterogeneous cell population. Two additional cell lines classified as TGr II (HCV-29 and Hu 609) were compared to two TGr III sublines (HCV-29T and Hu 609T, respectively) which arose by “spontaneous” transformation during propagation in vitro of the respective maternal TGr II-cell lines. One of these TGr II cell lines (HCV-29) originated from the histologically normal bladder mucosa obtained from a patient with a previous history of bladder papillomata treated with irradiation (Fogh, personal communication). The other TGr II cell line (Hu 609) was derived from the normal ureter of a patient with renal carcinoma.

In each of these 8 cell lines, the shape of 100 cells chosen at random were subjected to Fourier analysis of shape. Each of the particular harmonic amplitude values studied was used as an individual parameter for the evaluation of differences between compared cell lines, using Chi-Square test and discriminant analysis.

It was found that in two of four analysed pairs of cell lines, i.e. Hu 1703S (TGr I) vs Hu 1703He (TGr III) and HCV-29 (TGr II) vs HCV-29T (TGr III), the differences in cell shape between the two populations were very well pronounced, as was shown by several statistical parameters. In the two other pairs of cell lines, i.e. Hu 961b (TGr I) vs Hu 961a (TGr III) and Hu 609 (TGr II) vs Hu 609T (TGr III) significant differences in cell shape were also found, but they were less pronounced.

The conclusion is, that differences in cell shape in vitro may reveal the cellular heterogeneity of the original transitional cell carcinoma and/or the progression of in vitro propagated urothelial cells from one grade of transformation into another.

Keywords

Cell Shape Transitional Cell Carcinoma Chick Heart Transitional Cell Carcinoma Immortal Cell Line 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Anderson TW (1973) An assymetric expansion of the distribution of the studentized classification statistics Ann Statist 1:964–972Google Scholar
  2. Bryan GT (1983) Etiology an pathogenesis of bladder cancer. In: Bryan GT, Cohen SM (eds) The pathology of bladder cancer, vol 1. CRC Press, Boca Raton Florida, pp 1–9Google Scholar
  3. Bubenik J, Kieler J, Perlmann P, Paulie S, Koho H, Christensen B, Dienstbier Z, Kopřivová H, Pospišit J, Poučková P, Novak F, Dvořak P, Lauerová L, Kovařik J, Šimová J (1985) Monoclonal antibodies against human urinary bladder carcinoma: selectivy and utilization for gamma scientigraphy. Eur J Cancer Clin Oncol (in press)Google Scholar
  4. Burger M (1980) The cell surface and metastasis. In: Letnansky K (ed) Biology of the cancer cell. Proc V Meeting of EACR, Vienna 1979. Kugler Publications, Amsterdam, pp 193–208Google Scholar
  5. Christensen B, Kieler J, Vilien M, Don P, Wang CY, Wolf H (1984) A classification of human urothelial cells propagated in vitro. Anticancer Res 4:319–338Google Scholar
  6. Cooley WW, Lohnes PR (1971) Multivariant data analysis. Wiley Interscience, New YorkGoogle Scholar
  7. De Brabander M, De Mey J, Van de Veire R, Aerts F, Geuens G (1977) Microtubules in mammalian cell shape and surface modulation: an alternative hypothesis. Cell Biol Int Rep 1:453Google Scholar
  8. Dus D, Radzikowski C, Debray H, Montreuil J, Christensen B, Kieler J (1985) Lectin binding to non-malignant and malignant human uroepithelial cells in vitro. In: Bøg Hansen TC, Breborowicz J (eds) Proc VI Int Lectin Meeting, Poznan, Poland 1984. Lectins, biology, biochemistry, chemical biochemistry, vol 4 Walter de Gruyter, Berlin, pp 65–74Google Scholar
  9. Farber E, Cameron R (1980) The sequential analysis of cancer development. In: Klein G, Weinhouse S (eds) Adv Cancer Res, vol 31. Academic Press, New York, pp 125–226Google Scholar
  10. Foulds L (1969) Neoplastic development, vol 1. Academic Press, New YorkGoogle Scholar
  11. Foulds L (1975) Neoplastic development, vol 2. Academic Press, New YorkGoogle Scholar
  12. Friedman E, Verderame M, Winawer S, Pollack R (1984) Actin cytoskeletal organization loss in the benign-to-malignant tumor transition in cultured human colonic epithelial cells. Cancer Res 44:3040–3050Google Scholar
  13. Handleman SL, Sanford KK, Tarone RE, Parshad R (1977) The cytology of spontaneous neoplastic transformation in culture. In Vitro 13:526–536Google Scholar
  14. Hart IR, Fidler IJ (1981) The implications of tumor heterogeneity for studies on the biology and therapy of cancer metastasis. Biochim Biophys Acta 651:37–50Google Scholar
  15. Heppner GH (1984) Perspectives in cancer research, tumor heterogeneity Cancer Res 44:2259–2265Google Scholar
  16. Hicks RM, Chowaniec J (1978) Experimental induction, histology, and ultrastructure of hyperplasia and neoplasia of the urinary bladder epithelium. Int Rev Exp Pathol 18:199–280Google Scholar
  17. Hynes RO (1979) Proteins and glycoproteins. In: Hynes RO (ed) Surfaces of normal and malignant cells. John Wiley and Sons, New York, pp 103–148Google Scholar
  18. Keski-Oja J (1983) Microfilaments and intermediate filaments in epithelial cells transformed by murine sarcoma or leukemia viruses. Eur J Cell Biol 30:191–199Google Scholar
  19. Kieler J (1984) Invasiveness of transformed bladder epithelial cells. Cancer Metastasis Rev 3:265–296Google Scholar
  20. Kieler J, Ostrowski K, Strojny P, Rozycka M, Dziedzic-Goclawska A, Bulski W (1984) Fourier analysis of the shape of normal and transformed epithelial cells derived from human transitional epithelium. Histochemistry 81:119–128Google Scholar
  21. Kunze E (1979) Development of urinary bladder cancer in the rat. In: Grundman E (ed) Carcinogenesis. Current topics in pathology, vol 67. Springer, Berlin Heidelberg New York, pp 142–163Google Scholar
  22. Land H, Parada LF, Weinberg RA (1983) Cellular and multistep carcinogenesis. Science 222:772–778Google Scholar
  23. Lloyd CW, Smith CG, Woods A, Rees DA (1977) Mechanisms of cellular adhesion. II. The interplay between adhesion, the cytoskeleton and morphology in substrate-attached cells. Exp Cell Res 110:427–437Google Scholar
  24. Nicolson GL (1984) Cell surface molecules and tumor metastasis. Regulation of metastatic phenotypic diversity. Exp Cell Res 150:3–22Google Scholar
  25. Perbal B (1984) Transformation parameters expressed by tumorvirus transformed cells. In: Klein G (ed) Advances in viral oncology, vol 4. Raven Press, New York, pp 163–195Google Scholar
  26. Rohrschneider L, Rosok M, Shriver K (1982) Mechanism of transformation by Rous sarcoma virus: events within adhesion plaques. Cold Spring Harbor Symp Quant Biol 46:953–965Google Scholar
  27. Vilien M, Christensen B, Wolf H, Rasmussen F, Hou-Jensen C, Povlsen CO (1983) Comparative studies of normal “spontaneously” transformed and malignant human urothelium cells in vitro. Eur J Cancer Clin Oncol 19:775–789Google Scholar
  28. Willmott N, Simpson S (1983) Wheat germ agglutinin binding to cells derived from in vivo grown solid tumors. Anticancer Res 3:401–406Google Scholar
  29. Zahn CT, Roskies RZ (1972) Fourier descriptors for plane closed curves. IEEE Trans Comput 21:269–281Google Scholar

Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • K. Ostrowski
    • 1
  • A. Dziedzic-Goclawska
    • 2
  • P. Strojny
    • 1
  • W. Grzesik
    • 1
  • J. Kieler
    • 3
  • B. Christensen
    • 3
  • M. Mareel
    • 4
  1. 1.Department of HistologyMedical AcademyWarsawPoland
  2. 2.Department of TransplantologyMedical AcademyWarsawPoland
  3. 3.The Fibiger InstituteThe Danish Cancer SocietyCopenhagen ØDenmark
  4. 4.Department of Radiotherapy and Nuclear MedicineUniversity HospitalGentBelgium

Personalised recommendations