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Matrix models

Essential tools for microscopic cytochemical research

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Summary

An overview is given of the preparation and use of model systems for cytochemistry, dealing with quantitative as well as qualitative aspects.

Descriptions are given of the various possibilities to prepare cytochemical matrix models, ranging from macroscopic and microscopic films, to models with more cell-like dimensions as agarose beads, artificial cells and erythrocyte ghosts.

Such models allow the study of a large variety of cytochemical processes. Their potentialities are demonstrated in a number of specific applications, comprising: the study of the influence of fixation on cellular processes, reaction specificity and reaction kinetics, quality of reagents and biochemical calibration in cytochemical staining; factors influencing localization of the specific endproduct in enzyme cytochemistry; immunocytochemistry and hybridocytochemistry.

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Authors and Affiliations

  1. Department of Histochemistry and Cytochemistry, Leiden University, Wassenaarseweg 72, NL-2333 AL, Leiden, The Netherlands

    M. van der Ploeg & W. A. L. Duijndam

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  1. M. van der Ploeg
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  2. W. A. L. Duijndam
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In honour of Prof. P. van Duijn

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van der Ploeg, M., Duijndam, W.A.L. Matrix models. Histochemistry 84, 283–300 (1986). https://doi.org/10.1007/BF00482953

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