Fresenius' Zeitschrift für analytische Chemie

, Volume 335, Issue 4, pp 370–374 | Cite as

Spectral detection in thin-layer chromatography by linear photodiode array spectrometry

  • S. Bayerbach
  • G. Gauglitz
Original Papers Instrumental Optimizations in Spectrometry

Summary

A new method of spectral detection in thin-layer chromatography (TLC) is given. Spectral reflectance and fluorescence can be measured by use of fiber optics and a photodiode array. The possibilities of the method with respect to quantitative evaluation, background subtraction, and recognition of overlapped spots are demonstrated at examinations of dyes in comparison to conventional single wavelength detection. The apparatus, the experimental limitations, and future developments are discussed.

Keywords

Chromatography Physical Chemistry Analytical Chemistry Inorganic Chemistry Fiber Optic 

Spektrale Detektion in der Dünnschicht-Chromatographie durch lineare Photodiodenarray-Spektrometrie

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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • S. Bayerbach
    • 1
  • G. Gauglitz
    • 1
  1. 1.Institut für Physikalische und Theoretische ChemieTübingenFederal Republic of Germany

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