Speziesanalytik von Cadmium und Thallium in nativen Rapspflanzen (Brassica napus)

  • Klaus Günther
  • Fritz Umland
Environmental Analysis

Speciation of cadmium and thallium in native rape (Brassica napus)

Summary

The speciation of cadmium and thallium was studied in leaves from unpolluted rape of two different growth stages. In average the cytoplasmic fraction contained 37% of the whole cadmium or 84% of the whole thallium, respectively. Gel filtration of the cytoplasmic samples revealed a high (M>80,000 g/mol) and two low molecular weight cadmium species (M=4400 g/mol), but only one thallium species (M=3800 g/mol). No free ionic species have been detected. The determinations of metals were performed by ET-AAS, with a recovery between 60% and 90%. The low molecular weight species containing most of the cadmium was further purified by means of ion-exchange chromatography and gel filtration. The resulting strong anionic peptide showed a low absorption at 280 nm. The molar cadmium/peptide ratio was about 1/140. An amino acid analysis indicated no cysteine or methionine. Conclusively, the main cytoplasmic cadmium species in native rape neither belongs to the well known classes of sulphureous phytochelatins nor to the metallothioneins.

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Literatur

  1. 1.
    Fujita M, Kawanishi T (1987) Plant Cell Physiol 28:379–382Google Scholar
  2. 2.
    Robinson NJ, Jackson PJ (1986) Physiol Plant 67:499–506Google Scholar
  3. 3.
    Grill E, Winnacker EL, Zenk MH (1987) Proc Natl Acad Sci USA 84:439–443Google Scholar
  4. 4.
    Henze W, Umland F (1987) In: BrÄtter P, Schramel P (Hrsg) Trace element analytical chemistry in medicine and biology, vol 4. Walter de Gruyter, Berlin New York, S 501–507Google Scholar
  5. 5.
    Anonymus (1980) Umweltbelastung durch Thallium. Bericht der Landesanstalt für Immissionschutz EssenGoogle Scholar
  6. 6.
    Bradford MM (1976) Anal Biochem 72:248–254Google Scholar
  7. 7.
    Salomons W, Förstner U (1984) Metals in the hydrological cycle. Springer, Berlin Heidelberg New YorkGoogle Scholar
  8. 8.
    Merian E (1984) Metalle in der Umwelt. Verlag Chemie, WeinheimGoogle Scholar
  9. 9.
    Schoer J, Nagel U (1980) Naturwissenschaften 67:261–262Google Scholar
  10. 10.
    Henze W, Umland F (1987) Analytical Sciences 3:225–227Google Scholar

Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • Klaus Günther
    • 1
  • Fritz Umland
    • 1
  1. 1.Institut für Anorganische Chemie der UniversitÄtMünsterBundesrepublik Deutschland

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