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Enzymelektroden Zur Bestimmung von Lactat, Pyruvat und der Aktivität von Lactatdehydrogenase

Enzyme electrodes for lactate, pyruvate and lactate dehydrogenase activity determination

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  • Biochemisch-klinische Analysen
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Summary

Enzyme electrodes were assembled by coupling membrane-immobilized lactate monooxygenase and coimmobilized lactate monooxygenase and lactate dehydrogenase, respectively, to oxygen electrodes. The lactate monooxygenase sensor was used for lactate determination with the analyzer “Glukometer” as well as for sequential measurement of lactate and lactate dehydrogenase activity.

The bienzyme electrode is applicable for determining lactate and pyruvate in one measuring cycle. The operational parameters of the optimized sensors like measuring range, relative standard deviation, measuring frequency, and stability were studied. Determinations in biological fluids correlated well with spectrophotometric reference methods.

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Authors and Affiliations

  1. Kreiskrankenhaus Marienberg, Zentrallabor, DDR-9340, Marienberg, Deutsche Demokratische Republik

    D. Weigelt

  2. Akademie der Wissenschaften der DDR, Zentralinstitut für Molekularbiologie, Bereich Enzymologie, DDR-1115, Berlin-Buch, Deutsche Demokratische Republik

    F. Schubert & F. Scheller

Authors
  1. D. Weigelt
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  2. F. Schubert
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  3. F. Scheller
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Weigelt, D., Schubert, F. & Scheller, F. Enzymelektroden Zur Bestimmung von Lactat, Pyruvat und der Aktivität von Lactatdehydrogenase. Z. Anal. Chem. 328, 259–262 (1987). https://doi.org/10.1007/BF00481633

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  • DOI: https://doi.org/10.1007/BF00481633

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