Abstract
Pathogenic and non-pathogenic free-living amoebae from the Root Zone Method (RZM) of wastewater treatment were investigated. Ten reed beds planted on four different substrate types were compared for their efficiency of removal of free-living amoebae. Reed beds planted on coarse substrates generally gave the highest removal rates (up to 100%) while those planted in soil gave the lowest (60.4%). Possible reasons for differences in removal efficiencies are discussed. Thirteen species representing nine genera of free-living amoebae were isolated; these included three species of Acanthamoeba (A. astronyxis, A. polyphaga and A.rhysodes) all of which were pathogenic for mice. The implications for RZM design of the presence of potentially pathogenic free-living amoebae in the effluent are discussed.
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References
Červa, L.: 1973, Prog. Protozool., Proceedings of the 4th. International Congress on Protozoology, 2–9 September 1973, Clermont Ferrand, p. 83.
Cooper, P. F. and Findlater, B. C.: 1990, Constructed Wetlands in Water Pollution Control, Pergamon, Oxford.
Curds, C. R., Rivera, F., Ramírez, E., Warren, A., Bonilla, P., Calderón, A. Rodríguez, S., and Ortiz, R.: 1991, ‘Pathogenic and Free-Living Amoebae in an Activated Sludge System in London’, in Biological Approach to Sewage Treatment Processes: Current Status and Perspectives. Proceedings of International Symposium, October 15–17, 1990., Perugia, Italy, 1990, pp. 123–125.
De Jonckheere, J. F.: 1977, Appl. Environ. Microbiol. 33, 751.
De Jonckheere, J. F. and Van de Voorde, H.: 1977, Am. J. Trop. Med. Hyg. 26, 10.
Findlater, B. C., Hobson, J. A., and Cooper, P. F.: ‘Reed Bed Treatment Systems: Performance Evaluation’, in P. F. fnCooper and B. C. Findlater (eds.), Constructed Wetlands in Water Pollution Control, Pergamon, Oxfords.
Martinez, A. J.: 1985, Free-Living Amebas: Natural History, Prevention, Diagnosis, Pathology, and Treatment of Disease, CRC, Florida.
Page, F. C.: 1976, An Illustrated Guide to Freshwater and Soil Amoebae, Freshwater Biological Association Scientific Publication No. 34.
Page, F. C.: 1988, A New Key to Freshwater and Soil Gymnamoebae, Freshwater Biological Association, UK.
Parr, T. W.: 1989, ‘Survey of Reed Growth in UK Reed Bed Treatment Systems’, WRC/NERC Contract Report T02058el, Medmenham, UK.
Pussard, M. and Pons, R.: 1977, Protistologica 13, 557.
Rivera, F., Roy-Ocotla, G., Rosas, I., Ramírez, E., Bonilla, P., and Lares, F.: 1987, Environ. Res. 42, 149.
Sawyer, T. K.: 1989, Appl. Environ. Microbiol. 55, 1074.
Singh, B. N.: 1952, Phil. Trans Roy. Soc. Lond. B. 236, 405.
Singh, B. N.: 1975, Pathogenic and Non-Pathogenic Amoebae, Macmillan, London.
Singh, B. N. and Das, S. R.: 1972, Curr. Sci. 41, 277.
Thomas, H. A.: 1942, J. Am. Wat. Wks. Ass. 49, 1060.
Wellings, F. M., Amuso, P. T., Chang, S. L., and Lewis, A. L.: 1977, Appl. Environ. Microbiol. 34, 661.
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Rivera, F., Rodríguez, S., Warren, A. et al. An investigation of the pathogenic and non-pathogenic free-living amoebae from the root zone method of wastewater treatment. Water Air Soil Pollut 69, 93–98 (1993). https://doi.org/10.1007/BF00478350
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DOI: https://doi.org/10.1007/BF00478350