European Journal of Pediatrics

, Volume 126, Issue 4, pp 243–252 | Cite as

Comparison of RNA and DNA synthesis, spontaneous and PHA induced, between blood lymphoid cells of newborn infants, older infants, and adults

A study by scintillation counting and by autoradiography
  • Gregor Prindull
  • Brigitte Prindull
  • Werner Schröter
  • Joseph M. Yoffey
Original Investigations

Abstract

Spontaneous and PHA induced RNA and DNA synthesis was measured qualitatively by autoradiography and quantitatively by scintillation counting in blood lymphoid cells of newborn infants, older infants, and adults. Spontaneous RNA synthesis was found in transitional cells, large phagocytic lymphoid cells and lymphocytes. Transitional cells and phagocytic lymphoid cells also synthesized DNA spontaneously. Quantitatively, spontaneous RNA synthesis was active in both newborn infants and in adults, but significantly less so in older infants. PHA stimulation for 18 h increased RNA synthesis significantly in blood lymphoid cells of newborn infants and adults, and to a much lesser but still significant degree in older infants. Spontaneous DNA synthesis was significantly greater in newborn infants than in older infants and adults. PHA stimulation for 18 h had no effect on thymidine incorporation in any of the groups studied.

Key words

RNA DNA Lymphoid cells Newborn infants 

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Copyright information

© Springer-Verlag 1977

Authors and Affiliations

  • Gregor Prindull
    • 1
  • Brigitte Prindull
    • 1
  • Werner Schröter
    • 1
  • Joseph M. Yoffey
    • 2
  1. 1.Department of PediatricsUniversity of GöttingenGöttingenFederal Republic of Germany
  2. 2.The Department of AnatomyThe Hebrew University, Hadassah Medical SchoolJerusalemIsrael

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