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Evaluation of a new kit for the determination of 3-methoxy-4-hydroxymandelic acid (VMA) by liquid chromatography and electrochemical detection (LCEC)

Auswertung eines neuen Kits zur Bestimmung von 3-Methoxy-4-hydroxymandelsäure (VMA) durch Flüssig-Chromatographie mit elektrochemischer Detektion (LCEC)

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Zusammenfassung

Der neue Bio-Rad-Kit zur Bestimmung von VMA durch HPLC mit elektrochemischer Detektion wurde ausgewertet. Diese Bestimmung ist von Bedeutung im Zusammenhang mit der Tumordiagnose (Metabolit von Catecholaminen). Eine Einwegsäule wird zur Entfernung von störenden Substanzen benutzt. Das Eluat dieser Säule wird in das HPLC-System injiziert. Die Detektion erfolgt mit einem elektrochemischen Dualdetektor. Durch einen inneren Standard wird die Zuverlässigkeit der Bestimmungen erhöht. Der neue Kit ist in bezug auf Handhabung, Spezifität und Reproduzierbarkeit für den klinischen Routineeinsatz gut brauchbar.

Summary

The new Bio-Rad kit for the determination of 3-methoxy-4-hydroxymandelic acid by high-performance liquid chromatography and electrochemical detection has been evaluated.

The measurement of the major metabolite of the catecholamines is crucial for the diagnosis and the follow up of patient's tumors.

A disposable column is used for ion-exchange and ion-moderated partition to remove interfering compounds. The column eluate is injected into an HPLC system for separation and is monitored by a dual electrochemical detector. The reliability of the results are increased by the use of an internal standard. Easy to handle, specific and reproducible, this new kit successfully replaces the old one and seems to be reliable for routine clinical use.

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Authors and Affiliations

  1. Hôpital de Warquignies, 27, Rue des Chaufours, B-7360, Boussu, Belgium

    J. Dutrieu & Y. A. Delmotte

Authors
  1. J. Dutrieu
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  2. Y. A. Delmotte
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Dutrieu, J., Delmotte, Y.A. Evaluation of a new kit for the determination of 3-methoxy-4-hydroxymandelic acid (VMA) by liquid chromatography and electrochemical detection (LCEC). Z. Anal. Chem. 319, 70–73 (1984). https://doi.org/10.1007/BF00476233

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  • DOI: https://doi.org/10.1007/BF00476233

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