Fresenius' Zeitschrift für analytische Chemie

, Volume 331, Issue 1, pp 55–57 | Cite as

Characterisation of disaccharide fragments from the enzymatic digestion of heparin by liquid secondary ion mass spectrometry

  • Barbara E. Brandau
  • Petra Fonrobert
  • Michael Przybylski
  • Hermann J. Roth
Original Papers Biological Materials

Summary

Disaccharide fragments resulting from the enzymatic digestion of heparins with heparinase have been purified by gel filtration chromatography and directly analyzed by positive and negative ion liquid secondary ion mass spectrometry (LSIMS). Following the chromatographic purification from excess sodium salt, the mass spectra of di- and tetrasaccharide fragment mixtures enabled the identification of up to three covalently bound sulfate moieties per glycosaminoglycan-disaccharide unit, by means of their molecular ions, containing the corresponding alkali-counterions.

Keywords

Chromatography Filtration Mass Spectrum Heparin Enzymatic Digestion 

Charakterisierung von Disaccharidfragmenten aus dem enzymatischen Heparinaufschluß mit Hilfe der Flüssig-Sekundärionen-Massenspektrometrie

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Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • Barbara E. Brandau
    • 1
  • Petra Fonrobert
    • 2
  • Michael Przybylski
    • 2
  • Hermann J. Roth
    • 1
  1. 1.Institut für Pharmazeutische ChemieUniversität TübingenTübingenGermany
  2. 2.Institut für Organische ChemieUniversität MainzMainzGermany

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