Archives of Microbiology

, Volume 145, Issue 4, pp 408–410 | Cite as

Alginate biosynthesis by Pseudomonas aeruginosa: effect of arsenite and other metabolic inhibitors

  • P. C. Banerjee
Short Communication


Biosynthesis of alginic acid in presence of metabolic inhibitors by resting cells of mucoid Pseudomonas aeruginosa was studied. Among the inhibitors tested, arsenite exhibited very interesting results, while the others showed no remarkable-effect. Firstly, arsenite stopped alginate production from all the substrates during initial hours of incubation; secondly, degradation of newly synthesized alginates to smaller molecular weight fragments took place if it was added after a few hours of incubation with the substrate; and thirdly, uncontrolled synthesis of alginate started after several hours of inhibition. Presence of arsenite was needed for the initial inhibitory phase of alginate synthesis; but once the cells were capable of synthesizing alginate after initial hours of inhibition, arsenite may be omitted from the medium.

Key words

Alginate Arsenite Inhibition Stimulation Degradation Metabolic inhibitors Pyruvate Pseudomonas aeruginosa 


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Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • P. C. Banerjee
    • 1
  1. 1.Department of Microbiology and ImmunologyUniversity of Illinois, Health Sciences CenterChicagoUSA

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