Archives of Microbiology

, Volume 145, Issue 4, pp 347–352 | Cite as

The respiratory chain of the facultative thermophile, Bacillus coagulans

  • A. S. Ball
  • C. Edwards
Original Papers

Abstract

Two oxidases were found to be present in membranes from the facultative thermophile Bacillus coagulans grown at 55°C, compared to one in cells grown at 37°C. Cytochrome spectra and inhibitors of the respiratory chain identified them as cytochrome oxidases aa3 and d. Both were present in membranes from 55°C grown cells, but only cytochrome oxidase aa3 was found in membranes from 37°C grown cells. The presence of cytochrome d in 55°C grown cultures was found to be due to decreased oxygen tension and not to the high growth temperature. This was confirmed by (a) induction of cytochrome d at 37°C under conditions of oxygen limitation and (b) its repression at 55°C under conditions of high aeration and its subsequent induction on lowering the dissolved oxygen concentration in chemostat cultures. Two cytochromes bmax 558 and λmax 562) were present in both 37°C and 55°C grown cells. Results from the inhibition of substrate oxidation by membranes suggested different pathways of electron transport by the respiratory chain.

Key words

Bacillus coagulans Facultative thermophile Respiratory chain Inhibitors Cytochromes 

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Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • A. S. Ball
    • 1
  • C. Edwards
    • 1
  1. 1.Department of MicrobiologyUniversity of LiverpoolLiverpoolUK

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