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Archives of oto-rhino-laryngology

, Volume 244, Issue 5, pp 273–277 | Cite as

The postnatal development of stimulated deoxyglucose uptake into the mouse cochlea and the inferior colliculus

  • B. Canlon
  • M. Anniko
Article

Summary

The effect of acoustic stimulation on the postnatal development of deoxyglucose uptake into the mouse cochlea and the inferior colliculus was evaluated. Animals between postnatal day 4 and 20 were separated into four different groups depending on their age. Tritiated deoxyglucose was injected intraperitoneally into each animal and tracer uptake was quantitated by microdissection of the tissues and scintillation counting. Acoustic stimulation at a noise level of 100 dB (A) resulted in supra-normal levels of deoxyglucose uptake for all auditory tissues during postnatal days 13, 14, and 15. The lateral wall tissues, which are non-sensory and non-neuronal, also increased deoxyglucose uptake following acoustic stimulation in a manner that paralleled the uptake by the sensory structures. Serum radioactivity and glucose levels remained unchanged during postnatal development, with these parameters remaining stable with acoustic stimulation.

Key words

Acoustic stimulation Cochlea Deoxyglucose uptake Development Inferior colliculus 

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References

  1. 1.
    Anniko M, Bagger-Sjöbäck D (1984) The stria vascularis. In: Friedmann I, Ballantyne JC (eds) Ultrastructural atlas of the inner ear. Butterworths, London, pp 184–208Google Scholar
  2. 2.
    Anniko M, Wroblewski R (1981) Elemental composition of the developing inner ear. Ann Otol Rhinol Laryngol 90:25–32Google Scholar
  3. 3.
    Bosher SK, Warren RL (1971) A study of the electrochemistry and osmotic relationships of cochlear fluids in the neonatal rat to the time of development of the endocochlear potential. J Physiol 212:739–761Google Scholar
  4. 4.
    Canlon B, Schacht J (1983) Acoustic stimulation alters deoxyglucose uptake in the mouse cochlea and inferior colliculus. Hear Res 10:217–226Google Scholar
  5. 5.
    Canlon B, Takada A, Schacht J (1984) Glucose utilization in the auditory system: cochlear dysfunction and species differences. Comp Biochem Physiol 78A:43–47Google Scholar
  6. 6.
    Chou CS, Aberdeen GC (1981) The sensitive period for induction of susceptibility to audiogenic seizures by kanamycin. Arch Otorhinolaryngol 232:215–220Google Scholar
  7. 7.
    Chugani HT, Phelps ME (1986) Maturational changes in cerebral function in finants determined by a 18FDG positron emission tomography. Science 231:840–843Google Scholar
  8. 8.
    Falk SA, Cook RO, Haesman JK, Sanders GM (1974) Noise-induced inner ear damage in newborn and adult guinea pigs. Laryngoscope 84:444–453Google Scholar
  9. 9.
    Hebert R, Langlois JM, Dussault JH (1985) Permanent defects in rat peripheral auditory function following perinatal hypothyroidism: determination of a critical period. Dev Brain Res 23:161–170Google Scholar
  10. 10.
    Henry KR (1983) Lifelong susceptibility to acoustic trauma: changing patterns of cochlear damage over the lifespan of the mouse. Audiology 22:372–383Google Scholar
  11. 11.
    Henry KR, Chole RA, McGinn MD, Frush DP (1981) Increased ototoxicity in both young and old mice. Acta Otolaryngol (Stockh) 107:92–95Google Scholar
  12. 12.
    Kennedy C, Sakurada M, Shinohara M, Miyaoka M (1982) Local cerebral glucose utilization in the newborn macaque monkey. Ann Neurol 12:333–340Google Scholar
  13. 13.
    Kikuchi K, Hilding D (1965) The development of the organ of Corti in the mouse. Acta Otolaryngol (Stockh) 60:207–222Google Scholar
  14. 14.
    Kraus HJ, Aulbach-Kraus K (1981) Morphological changes in the cochlea of the mouse after the onset of hearing. Hear Res 4:89–102Google Scholar
  15. 15.
    Lenoir M, Pujol R (1980) Sensitive period to acoustic trauma in the rat pup cochlea. Acta Otolaryngol (Stockh) 89: 317–322Google Scholar
  16. 16.
    Lim DJ, Anniko M (1985) Developmental morphology of the inner ear in the mouse. A SEM observation. Acta Otolaryngol (Stockh) [Suppl] 422:1–69Google Scholar
  17. 17.
    Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275Google Scholar
  18. 18.
    McGinn MD, Willot JF, Henry KR (1973) Effects of conductive hearing loss on auditory evoked potentials and audiogenic seizures in mice. Nature 244:255–256Google Scholar
  19. 19.
    McNamara JO (1984) Kindling, an animal model of complex partial epilepsy. Ann Neurol 16:S72-S76Google Scholar
  20. 20.
    Mikaelian D, Ruben RJ (1985) Development of hearing in the normal CBA-J mouse. Acta Otolaryngol (Stockh) 59:451–461Google Scholar
  21. 21.
    Pujol R, Hilding D (1973) Anatomy and physiology of the onset of auditory function. Acta Otolaryngol (Stockh) 76: 1–10Google Scholar
  22. 22.
    Ryan AF (1984) Anatomical measures of physiological parameters in the cochlea. In: Berlin C (Ed) Recent advances in hearing science. College Hill, San Diego, pp 181–194Google Scholar
  23. 23.
    Ryan AF, Woolf NK, Sharp FR (1982) Functional ontogeny in the central auditory pathway of the mongolian gerbil. Exp Brain Res 47:428–436Google Scholar
  24. 24.
    Saunders JC, Chen CS (1982) Sensitive period of susceptibility to auditory trauma in mammals. Environ Health Perspect 44:63–66Google Scholar
  25. 25.
    Saunders JC, Bock GR, Chen CS, Gates GR (1972) Effects of priming for audiogenic seizures on cochlear and behavioral responses in BALB/c mice. Exp Neurol 36:426–436Google Scholar
  26. 26.
    Saunders JC, Bock GR, James R, Chen CS (1972) Effects of priming for audiogenic seizures on auditory evoked responses in the cochlear nucleus and inferior colliculus of BALB/c mice. Exp Neurol 37:388–394Google Scholar
  27. 27.
    Schacht J, Canlon B (1985) Noise-induced changes of cochlear energy metabolism. In: Drescher DG (Ed) Auditory biochemistry. Thomas, Springfield, pp 389–400Google Scholar
  28. 28.
    Shnerson A, Devigne C, Pujol R (1982) Age-related changes in the C57BL/6J mouse cochlea. II. Ultrastructual findings. Dev Brain Res 2:77–88Google Scholar
  29. 29.
    Sokoloff L, Reivich RM, Kennedy C, Des Rosiers MH, Patlack CS, Pettigrew KD, Sakurada O, Shinohara M (1977) The 14C-deoxyglucose method for measurement of local cerebral glucose utilization. J Neurochem 28:897–916Google Scholar
  30. 30.
    Uziel A, Romand R, Marot M (1979) Electrophysiological study of the ototoxicity of kanamycin during development in guinea pig. Hear Res 1:203–211Google Scholar
  31. 31.
    Willot JF, Shnerson A (1978) Rapid development of tuning characteristics of inferior colliculus neurons of mouse pups. Brain Res 148:230–233Google Scholar
  32. 32.
    Winkel S, Bonding P, Kildegard L, Roosen J (1978) Possible effects of kanamycin and incubation in newborn children with low birth weight. Acta Paediatr Scand 67:709–715Google Scholar

Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • B. Canlon
    • 1
  • M. Anniko
    • 2
  1. 1.Physiology Department IIKarolinska InstituteStockholmSweden
  2. 2.Department of Otorhinolaryngology and Head and Neck SurgeryUmeå University HosptialUmeåSweden

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