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Archives of oto-rhino-laryngology

, Volume 217, Issue 3, pp 321–330 | Cite as

Scanning electron microscopy of the nerves within the organ of Corti

  • Göran Bredberg
Article

Summary

A method of studying the innervation inside the organ of Corti is presented. Rabbits, guinea pigs and chinchillas were fixed with perfusion of the perilymphatic spaces. The cochleas were dissected according to the surface specimen technique and the specimens critical point dried, coated with gold and studied in the SEM. The final dissection into the planes of the fluid spaces of the organ of Corti was done in the dry state using especially sharpened watch makers forceps and razor blade knives. The course of the afferent and efferent innervation is described and differences between the species illustrated. Small high nerve endings on the outer hair cells degenerate after cutting the efferent nerve supply by dividing the vestibular nerve indicating their efferent nature.

Key words

Nerves Organ of Corti (guinea pig, rabbit, chinchilla) SEM 

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References

  1. Andersson, T. F.: Techniques for presentation of three dimensional structure in preparing specimens for the electron microscopy. Trans. N.Y. Acad. Sci. 13, 130 (1951)Google Scholar
  2. Bredberg, G.: Cellular pattern and nerve supply of the human organ of Corti. Acta Otolaryngol. (Stockh.), Suppl. 236, 1 (1968)Google Scholar
  3. Bredberg, G., Lindeman, H., Ades, H. W., West, R., Engström, H.: Scanning electron microscopy of the organ of Corti. Science 170, 861 (1970)Google Scholar
  4. Bredberg, G., Ades, H. W., Engström, H.: Scanning electron microscopy of the normal and pathologically altered organ of Corti. Acta Otolaryngol. (Stockh.), Suppl. 301, 3 (1973)Google Scholar
  5. Engström, B.: Scanning electron microscopy of the inner structure of the organ of Corti and its neural pathways. Acta Otolaryngol. (Stockh.), Suppl. 319, 57 (1974)Google Scholar
  6. Engström, H., Ades, H. W., Andersson, A.: Structural pattern of the organ of Corti. Stockholm: Almqvist and Wiksell 1966Google Scholar
  7. Engström, H., Ades, H. W., Bredberg, G.: Normal structure of the organ of Corti and the effect of noise-induced cochlear damage. In: Sensori-neural hearing loss. A Ciba Foundation Symposium (eds. G. E. W. Wolstenholm, Julie Knight), pp. 127–156. London: Churchill 1970Google Scholar
  8. Iurato, S.: Efferent fibers to the sensory cells of Corti's organ. Exp. Cell. Res. 27, 162 (1962)Google Scholar
  9. Kimura, R. S., Wersäll, J.: Termination of the olivo-cochlear bundle in relation to the outer hair cells of the organ of Corti in guinea pig. Acta Otolaryngol. (Stockh.) 55, 11 (1962)Google Scholar
  10. Morrison, D., Schindler, R. A., Wersäll, J.: A quantitative analysis of the afferent innervation of the organ of Corti in the guinea pig. Acta Otolaryngol. (Stockh.) 79, 11 (1975)Google Scholar
  11. Smith, C. A., Rasmussen, G. L.: Recent observations on the olivocochlear bundle. Ann. Otol. 72, 489 (1963)Google Scholar
  12. Spoendlin, H.: The organization of the cochlear receptor. Advanc. Oto-Rhino-Laryngol. (Basel) 13, 1 (1966)Google Scholar
  13. Spoendlin, H.: Innervation patterns in the organ of Corti of the cat. Acta Otolaryngol. (Stockh.) 67, 239 (1969)Google Scholar
  14. Spoendlin, H.: Structural basis of peripheral frequency analysis. In: Frequency analysis and periodicity detection in hearing (eds. R. Plomp, G. F. Smoorenburg), pp. 1–40. Leiden: Sijthoff 1970Google Scholar
  15. Spoendlin, H.: Degeneration behaviour of the cochlear nerve. Arch. klin. exp. Ohr.-, Nas.- u. Kehlk.-Heilk. 200, 275 (1971)Google Scholar
  16. Spoendlin, H., Gacek, R.: Electron microscopic study of the efferent and afferent innervation of the organ of Corti in the cat. Ann. Otol. 72, 660 (1963)Google Scholar
  17. Thalmann, R.: Quantitative histochemistry and cytochemistry of the ear. In: Handbook of audiology and vestibular research methods (eds. Smith, C. A., Vernon, J. A.), pp. 359–418. Springfield, Ill.: Thomas 1976Google Scholar
  18. Thalmann, R., Thalmann, I., Comegys, T. H.: Dissection and chemical analysis of substructures of the organ of Corti. Laryngoscope 80, 1619 (1970)Google Scholar

Copyright information

© Springer-Verlag 1977

Authors and Affiliations

  • Göran Bredberg
    • 1
  1. 1.Dept. of Otolaryngology, Akademiska sjukhusetUniversity of UppsalaUppsalaSweden

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