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Molecular Biology Reports

, Volume 16, Issue 3, pp 149–153 | Cite as

Methods of epitope mapping

  • I. Pettersson
Article

Concluding remarks

It is important to remember that the merits of the different approaches to epitope mapping should be judged against the purpose of the study. A peptide specifically recognized by nearly all sera containing a certain autoimmune specificity [20, 21, 26], would most likely be selected for the detection of the anti-linear/continuous epitope fraction among those autoantibodies and could be highly useful for diagnostic purposes. This is true even if a majority of the antibodies were directed against conformational/discontinuous protein epitopes. If the purpose is to study the induction or maintenance of the autoimmune response, one would also have to look at and account for the conformation-dependent autoantibodies. There is also the possibility that some pathogenic autoantibodies could constitute a small fraction requiring the complete nucleic acid-protein complex as an antigen. In that case, the ‘pathogenic’ epitope would not be identified nor mapped using the techniques discussed in this review.

Key words

autoantibody autoantigen B-cell epitope 

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References

  1. 1.
    Tan EM (1989) Adv. Immunol. 49: 93–151Google Scholar
  2. 2.
    Bautz EKF, Kalden JR, Homma M, Shulman LE & Tan EM (Eds) (1991) Molecular and Cell Biology of Autoantibodies and Autoimmunity Mol. Biol. Rep. 15: 95–216Google Scholar
  3. 3.
    Chambers JC & Keene JD (1985) Proc. Natl. Acad. Sci. USA 74: 5463–5467Google Scholar
  4. 4.
    Jerne NK (1960) Annu. Rev. Microbiol. 14: 341–358.Google Scholar
  5. 5.
    VanRegenmortel MHV, Briand JP, Muller S & Plaué S (1988) Synthetic polypeptides as antigens. Elsevier Scientific Publishers, AmsterdamGoogle Scholar
  6. 6.
    VanRegenmortel MHV (1989) Immunol. Today 10: 266–272Google Scholar
  7. 7.
    Laver WG, Air GM, Webster RG & Smith-Gill SJ (1990) Cell 61: 553–556Google Scholar
  8. 8.
    Geysen HM (1985) Immunol. Today 6: 364–369Google Scholar
  9. 9.
    Habets WJ, Sillekens PTG, Hoet MH, McAllister G, Lerner MR & vanVenrooij WJ (1989) Proc. Natl. Acad. Sci. USA 86: 4674–4678Google Scholar
  10. 10.
    Erlich HA (Ed) (1989) PCR Technology. Stockton Press, New YorkGoogle Scholar
  11. 11.
    Pisetsky DS & Groudier JP (1989) J. Immunol. 143: 3609–3613Google Scholar
  12. 12.
    Frorath B, Scanarini M, Netter HJ, Abney CC, Liedvogel B, Lakomek HJ & Northemann W (1991) Bio Techniques 11: 364–371.Google Scholar
  13. 13.
    Paxton H, Bendele T, O'Connor L & Haynes D (1990) Clin. Chem. 36: 792–797Google Scholar
  14. 14.
    Seelig HP, Ehrfeld H, Schroeter H, Heim C & Renz M (1991) J. Immunol. Methods 143: 11–24Google Scholar
  15. 15.
    Bini K, Chu J-L, Okolo C & Elkon K (1990) J. Clin. Invest. 85: 325–333Google Scholar
  16. 16.
    Cram DS, Fisicaro N, Coppel RL, Whittingham S & Harrison LC (1990) J. Immunol. 145: 630–635Google Scholar
  17. 17.
    Netter HJ, Guldner HH, Szotstecki C & Will H (1990) Scand. J. Immunol. 32: 163–176Google Scholar
  18. 18.
    Query CC, Bentley RC & Keene JD (1989) Cell 57: 89–101Google Scholar
  19. 19.
    Nagai K, Oubridge C, Jessen TH, Li J & Evans PR (1990) Nature 348: 515–520Google Scholar
  20. 20.
    Elkon KB, Skelly S, Parnassa AP, Danho W, Weissbach H & Brot N (1986) Proc. Natl. Acad. Sci. USA 83: 7419–7423Google Scholar
  21. 21.
    Hiness JJ, Danho W & Elkon KB (1991) Arthritis Rheum. 34: 572–579Google Scholar
  22. 22.
    St Clair EW, Kenan D, Burch JA, Keene JD & Pisetsky DS (1990) J. Immunol. 144: 3868–3876Google Scholar
  23. 23.
    Houghten RA (1985) Proc. Natl. Acad. Sci. USA 82: 5131–5135Google Scholar
  24. 24.
    Geysen HM, Meloen RH & Barteling SJ (1984) Proc. Natl. Acad. Sci. USA 81: 3998–4002Google Scholar
  25. 25.
    Scofield RH & Harley JB (1991) Proc. Natl. Acad. Sci. USA 88: 3343–3347Google Scholar
  26. 26.
    Barakat S, Briand JP, Abouaf N, VanRegenmortel MHV & Muller S (1991) Clin. exp. Immunol. 86: 71–78Google Scholar
  27. 27.
    Barakat S, Briand JP, Weber JC, VanRegenmortel MHV & Muller S (1990) Clin. Exp. Immunol. 81: 256–262Google Scholar
  28. 28.
    Berzotsky JA (1985) Science 229: 932–940Google Scholar

Copyright information

© Kluwer Academic Publishers 1992

Authors and Affiliations

  • I. Pettersson
    • 1
  1. 1.Department of Medical Cell Genetics, Medical Nobel InstitutetKarolinska InstitutetStockholmSweden

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