Advertisement

Archives of oto-rhino-laryngology

, Volume 244, Issue 3, pp 180–184 | Cite as

Light and electron microscopic studies of localized laryngeal amyloidosis

  • H. Nagata
  • T. Yoshihara
  • M. Nomoto
  • T. Kanda
  • T. Kaneko
  • Y. Yaku
Regular Contributions

Summary

We performed light and electron microscopic studies in a case of localized supraglottic laryngeal amyloidosis involving a 55-year-old woman. Tissue specimens were stained with hematoxylin and eosin, and alkaline Congo red with or without permanganate (KMnO4) treatment, and were also examined with peroxidase-antiperoxidase immunohistochemistry. Biopsy specimens from the epiglottis showed diffuse replacement of the subepithelial region by amyloid, which showed a typical green birefringence under polarized light. Tissue affinity for Congo red was persistent after the KMnO4 treatment. However, the tissue sections showed a positive reaction only to the anti-P component antibody. Electron microscopic studies revealed some disorders of the plasma cells, suggesting that these cells play an important role in focal production of amyloid fibrils.

Key words

Localized laryngeal amyloidosis Light microscopy Electron microscopy Immunohistochemistry 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Barnes EL, Zafar T (1977) Laryngeal amyloidosis. Clinicopathologic study of seven cases. Ann Otol 86:856–863Google Scholar
  2. 2.
    Cohen AX, Calkins E (1959) Electron microscopic observations on a fibrous component in amyloid of diverse origins. Nature 183:1202–1203Google Scholar
  3. 3.
    Crifò S (1973) Laryngeal idiopathic amyloidosis. ORL 35:117–126Google Scholar
  4. 4.
    Fujihara S, Glenner GG (1981) Primary localized amyloidosis of the genitourinary tract. Immunohistochemical study on eleven cases. Lab Invest 44:55–60Google Scholar
  5. 5.
    Fujihara S, Balow JE, Costa JC, Glenner GG (1980) Identification and classification of amyloid in formalin-fixed, Paraffin-embedded tissue sections by the unlabeled immunoperoxidase method. Lab Invest 43:358–365Google Scholar
  6. 6.
    Glenner GG (1980) A retrospective and prospective overview of the investigations on amyloid and amyloidosis — the β-fibrilloses. In: Glenner GG, Costa PP, Freitas AF (eds) Amyloid and amyloidosis. Excerpta Medica, Amsterdam, pp 3–13Google Scholar
  7. 7.
    Glenner GG (1980) Amyloid deposits and amyloidosis. The β-fibrilloses. N Engl J Med 302:1283–1292Google Scholar
  8. 8.
    Glenner GG, Ein D, Eanes ED, Bladen HA, Terry W, Page DL (1971) Creation of “amyloid” fibrils from Bence Jones proteins in vitro. Science 174:712–714Google Scholar
  9. 9.
    Graamans K, Lubsen H (1985) Clinical implications of laryngeal amyloidosis. J Laryngol Otol 99:617–623Google Scholar
  10. 10.
    Kaiser-Kupfer MI, McAdam KPWJ, Kuwabara T (1977) Localized amyloidosis of the orbit and upper respiratory tract. Am J Ophtalmol 84:721–728Google Scholar
  11. 11.
    McAlpine JC, Fuller AP (1964) Localized laryngeal amyloidosis. J Laryngol Otol 78:296–314Google Scholar
  12. 12.
    Montzouranis EC, Dowton SB, Whitehead AS, Edge MD, Bruns GAP, Colten HR (1985) Human amyloid P component. J Biol Chem 260:7752–7756Google Scholar
  13. 13.
    Page DL, Iserky C, Harada M, Glenner GG (1972) Immunoglobulin origin of localized nodular pulmonary amyloidosis. Res Exp Med 159:75–86Google Scholar
  14. 14.
    Puchtler H, Sweat F, Levine M (1972) On the binding of Congo red by amyloid. J Histochem Cytochem 10:355–364Google Scholar
  15. 15.
    Shindel J, Ben-Bossat H (1972) Amyloid tumor of the larynx. Case report with electron microscope study. Ann Otol 81:438–443Google Scholar
  16. 16.
    Shtrasburg S, Pras M, Langeviych P, Gal R (1982) Demonstration of AA-protein in Formalin-fixed, paraffin-embedded tissues. Am J Pathol 106:141–144Google Scholar
  17. 17.
    Wright JR, Calkins E, Humphrey RL (1977) Potassium permanganate reaction in amyloidosis. Lab Invest 36:274–281Google Scholar

Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • H. Nagata
    • 1
  • T. Yoshihara
    • 1
  • M. Nomoto
    • 1
  • T. Kanda
    • 1
  • T. Kaneko
    • 1
  • Y. Yaku
    • 2
  1. 1.Department of Otorhinolaryngology, School of MedicineChiba UniversityChibaJapan
  2. 2.Department of Head and Neck SurgeryTochigi Prefectural Cancer CenterUtsunomiyaJapan

Personalised recommendations